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Urinary excretion of neutrophil gelatinase-associated lipocalin in diabetic rats.
Arellano-Buendía, Abraham Said; García-Arroyo, Fernando Enrique; Cristóbal-García, Magdalena; Loredo-Mendoza, María Lilia; Tapia-Rodríguez, Edilia; Sánchez-Lozada, Laura Gabriela; Osorio-Alonso, Horacio.
Afiliação
  • Arellano-Buendía AS; Renal Pathophysiology Laboratory, Department of Nephrology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, DF, Mexico.
  • García-Arroyo FE; Renal Pathophysiology Laboratory, Department of Nephrology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, DF, Mexico.
  • Cristóbal-García M; Renal Pathophysiology Laboratory, Department of Nephrology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, DF, Mexico.
  • Loredo-Mendoza ML; Histopathology Laboratory, Research Subdivision, School of Medicine, Universidad Panamericana, Donatello 43, 03910 México City, DF, Mexico.
  • Tapia-Rodríguez E; Renal Pathophysiology Laboratory, Department of Nephrology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, DF, Mexico.
  • Sánchez-Lozada LG; Renal Pathophysiology Laboratory, Department of Nephrology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, DF, Mexico.
  • Osorio-Alonso H; Renal Pathophysiology Laboratory, Department of Nephrology, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano 1, Sección XVI, Tlalpan, 14080 México City, DF, Mexico.
Oxid Med Cell Longev ; 2014: 961326, 2014.
Article em En | MEDLINE | ID: mdl-25243053
UNLABELLED: Recent studies suggest that tubular damage precedes glomerular damage in the progression of diabetic nephropathy. Therefore, we evaluated oxidative stress and urinary excretion of tubular proteins as markers of tubular dysfunction. METHODS: Diabetes was induced in rats by streptozotocin administration (50 mg/kg). Oxidative stress was assessed by measuring the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD); additionally, expression levels of 3-nitrotyrosine (3-NT), 4-hydroxynonenal (4-HNE), and oxidized protein (OP) were quantified. Whole glomerular filtration rate (GFR) was measured. Urinary excretion of neutrophil gelatinase-associated lipocalin (uNGAL), osteopontin (uOPN), and N-acetyl-ß-D-glucosaminidase (uNAG) was also determined. RESULTS: Diabetic rats showed an increase in uNGAL excretion 7 days following induction of diabetes. Diuresis, proteinuria, albuminuria, creatinine clearance, and GFR were significantly increased by 30 days after induction. Furthermore, there was an increase in both CAT and SOD activity, in addition to 3-NT, 4-HNE, and OP expression levels. However, GPx activity was lower. Serum levels of NGAL and OPN, as well as excretion levels of uNGAL, uOPN, and uNAG, were increased in diabetics. Tubular damage was observed by 7 days after diabetes induction and was further aggravated by 30 days after induction. CONCLUSION: The tubular dysfunction evidenced by urinary excretion of NGAL precedes oxidative stress during diabetes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Fase Aguda / Proteínas Proto-Oncogênicas / Diabetes Mellitus Experimental / Nefropatias Diabéticas / Lipocalinas Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Oxid Med Cell Longev Assunto da revista: METABOLISMO Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Fase Aguda / Proteínas Proto-Oncogênicas / Diabetes Mellitus Experimental / Nefropatias Diabéticas / Lipocalinas Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Oxid Med Cell Longev Assunto da revista: METABOLISMO Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos