Molecular detection of Brucella species in patients suspicious of Brucellosis from Zanjan, Iran.

Garshasbi, Maryam; Ramazani, Ali; Sorouri, Rahim; Javani, Siamak; Moradi, Soheila

Garshasbi, Maryam; Ramazani, Ali; Sorouri, Rahim; Javani, Siamak; Moradi, Soheila.

Afiliação

Garshasbi M; Department of Microbiology Faculty of Medical and Basic Sciences Islamic Azad University Zanjan Iran Department of Microbiology, Faculty of Medical and Basic Sciences, Islamic Azad University, Zanjan, Iran.
Ramazani A; Biotechnology Department, School of Pharmacy Zanjan University of Medical Sciences Zanjan Iran Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Sorouri R; Molecular Biology Research Center Baqiyatallah University of Medical Sciences Tehran Iran Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Javani S; Biotechnology Department, School of Pharmacy Zanjan University of Medical Sciences Zanjan Iran Biotechnology Department, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
Moradi S; Department of Microbiology Faculty of Medical and Basic Sciences Islamic Azad University Zanjan Iran Department of Microbiology, Faculty of Medical and Basic Sciences, Islamic Azad University, Zanjan, Iran.

Braz J Microbiol ; 45(2): 533-8, 2014.

Article em En | MEDLINE | ID: mdl-25242938

RESUMO

Brucella is an intracellular pathogen capable of infecting animals and humans. The aim of this study was to identify Brucella spp in sera of high risk individuals by a polymerase chain reaction (PCR)-based method. A total of 180 patients suspected to have Brucellosis were examined by serological tests. To establish a PCR protocol for diagnosis of active brucellosis, DNA was extracted from the serum samples by using a commercial kit. PCR amplification was done for detection of Brocella DNA using BCSP31 target gene and IS711 locus. The PCR assay showed that an amplicon of 223 bp was obtained in 73.8% (133/180) of the tested sera using primers (B4/B5) derived from a gene encoding the 31-kDa Brucella abortus antigen. In another PCR, an amplicon of 498 bp was obtained in 63.8% (115/180) of the samples using Brucella abortus-specific primers derived from a locus adjacent to the 3'-end of IS711, and also an amplicon of 731 bp was produced in 4.4% (8/180) of the tested samples using Brucella melitensis-specific primers. When the Wright method was used as a gold standard, the sensitivity and specificity of the PCR technique for genus identification were found to be 96 and 80.7%, respectively. However, the sensitivity value obtained with the species-specific PCR method was 82%, and specificity was similar to that previous reported. This is the first report of a high frequency of Brucella abortus in patients suspicious of Brucellosis from the Zanjan province.

Assuntos

Brucella abortus/isolamento & purificação; Brucella melitensis/isolamento & purificação; Brucelose/diagnóstico; Técnicas de Diagnóstico Molecular/métodos; Reação em Cadeia da Polimerase/métodos; Soro/microbiologia; Adolescente; Adulto; Idoso; Animais; Antígenos de Bactérias/genética; Proteínas de Bactérias/genética; Brucella abortus/genética; Brucella melitensis/genética; Elementos de DNA Transponíveis; DNA Bacteriano/genética; DNA Bacteriano/isolamento & purificação; Feminino; Humanos; Irã (Geográfico); Masculino; Pessoa de Meia-Idade; Sensibilidade e Especificidade; Adulto Jovem

Palavras-chave

Brucella abortus; PCR; human Brucellosis; serologic tests

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Brucella abortus / Brucelose / Reação em Cadeia da Polimerase / Brucella melitensis / Técnicas de Diagnóstico Molecular / Soro Tipo de estudo: Diagnostic_studies / Evaluation_studies / Guideline / Prognostic_studies Limite: Adolescent / Adult / Aged / Animals / Female / Humans / Male / Middle aged País/Região como assunto: Asia Idioma: En Revista: Braz J Microbiol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil

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