A simple mathematical model based on biomarkers in stress-resistant catfish species, Sciades herzbergii (Pisces, Ariidae), in São Marcos Bay, Brazil.
Appl Biochem Biotechnol
; 174(7): 2380-91, 2014 Dec.
Article
em En
| MEDLINE
| ID: mdl-25227683
We present a refinement of our model describing the association between enzyme activity and histopathological lesions in the catfish, Sciades herzbergii from a polluted port. The fish were sampled from a port known to be contaminated with heavy metals and organic compounds and from a natural reserve in São Marcos Bay, Brazil. Two biomarkers, hepatic glutathione S-transferase (GST) activity and histopathological lesions, in gills and liver tissue were measured. The values for GST activity were modeled with the occurrence of branchial and hepatic lesions by fitting a third-order polynomial. Results from the mathematical model indicate that GST activity has a strong polynomial relationship with the occurrence of branchial and hepatic lesions in both wet and the dry seasons but only at the polluted port site. The model developed in this study indicates that branchial and hepatic lesions are initiated when GST activity reaches 2.17 µmol min(-1) mg protein(-1). Beyond this limit, GST activity decreased to very low levels and irreversible histopathological lesions occurred. This mathematical model based on two biomarkers (histopathological lesions and enzyme activity) in catfish provides a realistic approach to analyze stress induced by contaminants.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
/
Poluentes Químicos da Água
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Poluição Química da Água
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Peixes-Gato
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Proteínas de Peixes
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Glutationa Transferase
/
Modelos Biológicos
Limite:
Animals
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Appl Biochem Biotechnol
Ano de publicação:
2014
Tipo de documento:
Article
País de publicação:
Estados Unidos