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Isolation and characterization of 32 microsatellite loci for topmouth culter (Culter alburnus Basilewsky).
Liu, S-L; Gu, Z-M; Jia, Y-Y; Zhao, J-L; Jiang, W-P; Li, Q; Li, F.
Afiliação
  • Liu SL; Zhejiang Institute of Freshwater Fisheries, HuZhou, China.
  • Gu ZM; Zhejiang Institute of Freshwater Fisheries, HuZhou, China guzhimin2006@163.com.
  • Jia YY; Zhejiang Institute of Freshwater Fisheries, HuZhou, China.
  • Zhao JL; Key Laboratory of Freshwater Fisheries Germplasm Resource, Shangai Ocean University, Ministry of Agriculture, Shanghai, China.
  • Jiang WP; Zhejiang Institute of Freshwater Fisheries, HuZhou, China.
  • Li Q; Zhejiang Institute of Freshwater Fisheries, HuZhou, China.
  • Li F; Zhejiang Institute of Freshwater Fisheries, HuZhou, China.
Genet Mol Res ; 13(3): 7480-3, 2014 Sep 12.
Article em En | MEDLINE | ID: mdl-25222247
The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA)12 and (GA)12 probes. The variability of these microsatellites was tested on 30 individuals cultured. The average allele number was 6.6 per locus, ranging from 3 to 12. The observed heterozygosity was from 0.4667 to 0.9000, and the expected heterozygosity was from 0.6163 to 0.9085. After using Bonferroni's correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci, but deviations from Hardy-Weinberg equilibrium were found in 3 loci. These microsatellites can be used to study QTL of economic importance, population genetic diversity and the construction of genetic maps for C. alburnus in the future.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições de Microssatélites / Loci Gênicos / Peixes Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Repetições de Microssatélites / Loci Gênicos / Peixes Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil