Your browser doesn't support javascript.
loading
Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds.
Evaristo, Thaiane Cristine; CruzAlves, Flávia Cilene Maciel da; Moroz, Andrei; Mion, Woner; Acorci-Valério, Michele Janegitz; Felisbino, Sérgio Luis; Rossi-Ferreira, Rosana; Ruiz Júnior, Raul Lopes; Deffune, Elenice.
Afiliação
  • Evaristo TC; Department of Surgery and Orthopedy, Medical School, Paulista State University, Botucatu, SP, Brazil.
  • CruzAlves FC; Hermínio Ometto University Center, Araras, SP, Brazil.
  • Moroz A; Department of Bioprocess and Biotechnology, School of Pharmaceutical Sciences, UNESP, Araraquara, SP, Brazil.
  • Mion W; Blood Transfusion Center, Medical School, UNESP, Botucatu, SP, Brazil.
  • Acorci-Valério MJ; Department of Health Science, Paulista University, Bauru, SP, Brazil.
  • Felisbino SL; Department of Morphology, Bioscience Institute, UNESP, Botucatu, SP, Brazil.
  • Rossi-Ferreira R; Department of Science Biology, Faculty of Science, UNESP, Bauru, SP, Brazil.
  • Ruiz Júnior RL; Department of Surgery and Orthopedy, Medical School, UNESP, Botucatu, SP, Brazil.
  • Deffune E; Department of Urology, Medical School, UNESP, Botucatu, SP, Brazil.
Acta Cir Bras ; 29(8): 485-92, 2014 Aug.
Article em En | MEDLINE | ID: mdl-25140589
PURPOSE: To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal. METHODS: This study was performed with New Zealand adult rabbits weighing 3.0 - 4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase). RESULTS: Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present. CONCLUSION: The light-emitting diode is a promising tool for decellularization of soft tissues.
Assuntos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traqueia / Engenharia Tecidual / Alicerces Teciduais / Luz Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: Acta Cir Bras Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traqueia / Engenharia Tecidual / Alicerces Teciduais / Luz Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Revista: Acta Cir Bras Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil