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Comparison of fractal dimension and Shannon entropy in myocytes from rats treated with histidine-tryptophan-glutamate and histidine-tryptophan cetoglutarate.
Oliveira, Marcos Aurélio Barboza de; Brandi, Antônio Carlos; Santos, Carlos Alberto dos; Botelho, Paulo Henrique Husseni; Cortez, José Luís Lasso; Godoy, Moacir Fernandes de; Braile, Domingo Marcolino.
Afiliação
  • Oliveira MA; Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
  • Brandi AC; Hospital de Base São José do Rio Preto, São José do Rio Preto, SP, Brasil.
  • Santos CA; Hospital de Base São José do Rio Preto, São José do Rio Preto, SP, Brasil.
  • Botelho PH; Hospital de Base São José do Rio Preto, São José do Rio Preto, SP, Brasil.
  • Cortez JL; Santa Casa Votuporanga, Votuporanga, SP, Brasil.
  • Godoy MF; Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brasil.
  • Braile DM; Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, Brasil.
Rev Bras Cir Cardiovasc ; 29(2): 156-62, 2014.
Article em En, Pt | MEDLINE | ID: mdl-25140464
INTRODUCTION: Solutions that cause elective cardiac arrest are constantly evolving, but the ideal compound has not yet been found. The authors compare a new cardioplegic solution with histidine-tryptophan-glutamate (Group 2) and other one with histidine-tryptophan-cetoglutarate (Group 1) in a model of isolated rat heart. OBJECTIVE: To quantify the fractal dimension and Shannon entropy in rat myocytes subjected to cardioplegia solution using histidine-tryptophan with glutamate in an experimental model, considering the caspase markers, IL-8 and KI-67. METHODS: Twenty male Wistar rats were anesthetized and heparinized. The chest was opened, the heart was withdrawn and 40 ml/kg of cardioplegia (with histidine-tryptophan-cetoglutarate or histidine-tryptophan-glutamate solution) was infused. The hearts were kept for 2 hours at 4ºC in the same solution, and thereafter placed in the Langendorff apparatus for 30 min with Ringer-Locke solution. Analyzes were performed for immunohistochemical caspase, IL-8 and KI-67. RESULTS: The fractal dimension and Shannon entropy were not different between groups histidine-tryptophan-glutamate and histidine-tryptophan-acetoglutarate. CONCLUSION: The amount of information measured by Shannon entropy and the distribution thereof (given by fractal dimension) of the slices treated with histidine-tryptophan-cetoglutarate and histidine-tryptophan-glutamate were not different, showing that the histidine-tryptophan-glutamate solution is as good as histidine-tryptophan-acetoglutarate to preserve myocytes in isolated rat heart.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Soluções Cardioplégicas / Ácido Glutâmico / Miócitos Cardíacos / Parada Cardíaca Induzida Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En / Pt Revista: Rev Bras Cir Cardiovasc Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Soluções Cardioplégicas / Ácido Glutâmico / Miócitos Cardíacos / Parada Cardíaca Induzida Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En / Pt Revista: Rev Bras Cir Cardiovasc Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil