Your browser doesn't support javascript.
loading
Rmcystatin3, a cysteine protease inhibitor from Rhipicephalus microplus hemocytes involved in immune response.
Lu, Stephen; Soares, Tatiane S; Vaz Junior, Itabajara S; Lovato, Diogo V; Tanaka, Aparecida S.
Afiliação
  • Lu S; Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil.
  • Soares TS; Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil.
  • Vaz Junior IS; Centro de Biotecnologia do Estado do Rio Grande do Sul, UFRGS, Porto Alegre, Brazil.
  • Lovato DV; Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil.
  • Tanaka AS; Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, 04044-020 São Paulo, SP, Brazil. Electronic address: tanaka.bioq@epm.br.
Biochimie ; 106: 17-23, 2014 Nov.
Article em En | MEDLINE | ID: mdl-25064361
The Rhipicephalus microplus tick is responsible for losses in the livestock production estimated in 2 billions USD. Despite its economical importance the knowledge in tick's physiology is sparse. In order to contribute to this scenario we describe the characterization of a cysteine proteinase inhibitor named Rmcystatin-3. Purified recombinant Rmcystatin-3 was able to inhibit cathepsin L (Ki = 2.5 nM), BmCl1 (Ki = 1.8 nM) and cathepsin B (Ki = 136 nM). Western blot and quantitative PCR analysis revealed the presence of Rmcystatin-3 in fat body, salivary gland but mainly in hemocytes. The mRNA levels of Rmcystatin-3 during bacterial challenge are drastically down-regulated. In order to define the Rmcystatin-3 possible role in tick immunity, the cystatin gene was knockdown by RNA interference with and without Escherichia coli infection. Our results showed that the Rmcystatin-3 silenced group was more immune competent to control bacterial infection than the group injected with non-related dsRNA. Taking together, our data strongly suggested an important role of Rmcystatin-3 in tick immunity.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inibidores de Cisteína Proteinase / Rhipicephalus / Resistência à Doença / Hemócitos Limite: Animals Idioma: En Revista: Biochimie Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inibidores de Cisteína Proteinase / Rhipicephalus / Resistência à Doença / Hemócitos Limite: Animals Idioma: En Revista: Biochimie Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: França