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Reliability of a rapid hematology stain for sputum cytology.
Gonçalves, Jéssica; Pizzichini, Emilio; Pizzichini, Marcia Margaret Menezes; Steidle, Leila John Marques; Rocha, Cristiane Cinara; Ferreira, Samira Cardoso; Zimmermann, Célia Tânia.
Afiliação
  • Gonçalves J; Clinical Analysis Department, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Pizzichini E; University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Pizzichini MM; University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Steidle LJ; Department of Clinical Medicine, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Rocha CC; Center for Research on Asthma and Airway Inflammation, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Ferreira SC; Clinical Analysis Department, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.
  • Zimmermann CT; Clinical Analysis Department, Laboratory Technician, University Hospital, Federal University of Santa Catarina, Florianópolis, Brazil.
J Bras Pneumol ; 40(3): 250-8, 2014.
Article em En, Pt | MEDLINE | ID: mdl-25029648
OBJECTIVE: To determine the reliability of a rapid hematology stain for the cytological analysis of induced sputum samples. METHODS: This was a cross-sectional study comparing the standard technique (May-Grünwald-Giemsa stain) with a rapid hematology stain (Diff-Quik). Of the 50 subjects included in the study, 21 had asthma, 19 had COPD, and 10 were healthy (controls). From the induced sputum samples collected, we prepared four slides: two were stained with May-Grünwald-Giemsa, and two were stained with Diff-Quik. The slides were read independently by two trained researchers blinded to the identification of the slides. The reliability for cell counting using the two techniques was evaluated by determining the intraclass correlation coefficients (ICCs) for intraobserver and interobserver agreement. Agreement in the identification of neutrophilic and eosinophilic sputum between the observers and between the stains was evaluated with kappa statistics. RESULTS: In our comparison of the two staining techniques, the ICCs indicated almost perfect interobserver agreement for neutrophil, eosinophil, and macrophage counts (ICC: 0.98-1.00), as well as substantial agreement for lymphocyte counts (ICC: 0.76-0.83). Intraobserver agreement was almost perfect for neutrophil, eosinophil, and macrophage counts (ICC: 0.96-0.99), whereas it was moderate to substantial for lymphocyte counts (ICC = 0.65 and 0.75 for the two observers, respectively). Interobserver agreement for the identification of eosinophilic and neutrophilic sputum using the two techniques ranged from substantial to almost perfect (kappa range: 0.91-1.00). CONCLUSIONS: The use of Diff-Quik can be considered a reliable alternative for the processing of sputum samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Corantes Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En / Pt Revista: J Bras Pneumol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Escarro / Corantes Tipo de estudo: Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En / Pt Revista: J Bras Pneumol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil