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Detection of sickle cell hemoglobin in Haiti by genotyping and hemoglobin solubility tests.
Carter, Tamar E; von Fricken, Michael; Romain, Jean R; Memnon, Gladys; St Victor, Yves; Schick, Laura; Okech, Bernard A; Mulligan, Connie J.
Afiliação
  • Carter TE; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
  • von Fricken M; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
  • Romain JR; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
  • Memnon G; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
  • St Victor Y; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
  • Schick L; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
  • Okech BA; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
  • Mulligan CJ; Genetics and Genomics Program, University of Florida, Gainesville, Florida; Department of Anthropology, University of Florida, Gainesville, Florida; Genetics Institute, University of Florida, Gainesville, Florida; Emerging Pathogens Institute, University of Florida, Gainesville, Florida; Department
Am J Trop Med Hyg ; 91(2): 406-11, 2014 Aug.
Article em En | MEDLINE | ID: mdl-24957539
Sickle cell disease is a growing global health concern because infants born with the disorder in developing countries are now surviving longer with little access to diagnostic and management options. In Haiti, the current state of sickle cell disease/trait in the population is unclear. To inform future screening efforts in Haiti, we assayed sickle hemoglobin mutations using traditional hemoglobin solubility tests (HST) and add-on techniques, which incorporated spectrophotometry and insoluble hemoglobin separation. We also generated genotype data as a metric for HST performance. We found 19 of 202 individuals screened with HST were positive for sickle hemoglobin, five of whom did not carry the HbS allele. We show that spectrophotometry and insoluble hemoglobin separation add-on techniques could resolve false positives associated with the traditional HST approach, with some limitations. We also discuss the incorporation of insoluble hemoglobin separation observation with HST in suboptimal screening settings like Haiti.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hemoglobina Falciforme / Genótipo / Anemia Falciforme Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Humans / Infant / Middle aged País/Região como assunto: Haiti Idioma: En Revista: Am J Trop Med Hyg Ano de publicação: 2014 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Hemoglobina Falciforme / Genótipo / Anemia Falciforme Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Adolescent / Adult / Aged / Aged80 / Child / Child, preschool / Humans / Infant / Middle aged País/Região como assunto: Haiti Idioma: En Revista: Am J Trop Med Hyg Ano de publicação: 2014 Tipo de documento: Article País de publicação: Estados Unidos