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Protein kinase A mediates adenosine A2a receptor modulation of neurotransmitter release via synapsin I phosphorylation in cultured cells from medulla oblongata.
Matsumoto, Joao Paulo Pontes; Almeida, Marina Gomes; Castilho-Martins, Emerson Augusto; Costa, Maisa Aparecida; Fior-Chadi, Debora Rejane.
Afiliação
  • Matsumoto JP; Department of Physiology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Almeida MG; Department of Physiology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Castilho-Martins EA; Department of Physiology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Costa MA; Department of Physiology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, Brazil.
  • Fior-Chadi DR; Department of Physiology, Institute of Biosciences, University of Sao Paulo, Sao Paulo, Brazil. Electronic address: drfchadi@usp.br.
Neurosci Res ; 85: 1-11, 2014 Aug.
Article em En | MEDLINE | ID: mdl-24912137
Synaptic transmission is an essential process for neuron physiology. Such process is enabled in part due to modulation of neurotransmitter release. Adenosine is a synaptic modulator of neurotransmitter release in the Central Nervous System, including neurons of medulla oblongata, where several nuclei are involved with neurovegetative reflexes. Adenosine modulates different neurotransmitter systems in medulla oblongata, specially glutamate and noradrenaline in the nucleus tractussolitarii, which are involved in hypotensive responses. However, the intracellular mechanisms involved in this modulation remain unknown. The adenosine A2a receptor modulates neurotransmitter release by activating two cAMP protein effectors, the protein kinase A and the exchange protein activated by cAMP. Therefore, an in vitro approach (cultured cells) was carried out to evaluate modulation of neurotransmission by adenosine A2a receptor and the signaling intracellular pathway involved. Results show that the adenosine A2a receptor agonist, CGS 21680, increases neurotransmitter release, in particular, glutamate and noradrenaline and such response is mediated by protein kinase A activation, which in turn increased synapsin I phosphorylation. This suggests a mechanism of A2aR modulation of neurotransmitter release in cultured cells from medulla oblongata of Wistar rats and suggest that protein kinase A mediates this modulation of neurotransmitter release via synapsin I phosphorylation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapsinas / Proteínas Quinases Dependentes de AMP Cíclico / Neurotransmissores / Receptor A2A de Adenosina / Neurônios Limite: Animals / Humans Idioma: En Revista: Neurosci Res Assunto da revista: NEUROLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sinapsinas / Proteínas Quinases Dependentes de AMP Cíclico / Neurotransmissores / Receptor A2A de Adenosina / Neurônios Limite: Animals / Humans Idioma: En Revista: Neurosci Res Assunto da revista: NEUROLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda