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Detection of anti-nuclear antibodies by indirect immunofluorescence on HEp-2 cells: setting the appropriate screening dilution for the diagnosis of autoimmune rheumatic diseases.
Brito, Fabiano de Almeida; Santos, Silvana Maria Elói; Ferreira, Gilda Aparecida; Pedrosa, William; Gradisse, Janaina; Costa, Lara Cristina; Neves, Suzane Pretti Figueiredo.
Afiliação
  • Brito Fde A; Instituto Hermes Pardini, Belo Horizonte, Belo Horizonte, MG, Brasil.
  • Santos SM; Departamento de Propedêutica Complementar, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
  • Ferreira GA; Departamento do Aparelho Locomotor, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
  • Pedrosa W; Instituto Hermes Pardini, Belo Horizonte, Belo Horizonte, MG, Brasil.
  • Gradisse J; Instituto Hermes Pardini, Belo Horizonte, Belo Horizonte, MG, Brasil.
  • Costa LC; Instituto Hermes Pardini, Belo Horizonte, Belo Horizonte, MG, Brasil.
  • Neves SP; Departamento de Propedêutica Complementar, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
Rev Bras Reumatol ; 54(1): 13-20, 2014.
Article em En, Pt | MEDLINE | ID: mdl-24878786
OBJECTIVE: To establish the abnormal title and the appropriate screening dilution for ANA (antinuclear antibodies) test by indirect immunofluorescence on HEp-2 cells (ANA HEp-2). METHODS: An analysis of ANA Hep-2 in serum samples from 126 healthy individuals was performed. The samples were screened at a dilution of 1:80, and those positive were diluted to the title of 1:5120. The abnormal title of ANA was defined as that corresponding to the 95th percentile of the test in this population. The sensitivity of the different titles of antinuclear antibodies was determined in a group of 136 patients with a diagnosis of autoimmune rheumatic disease, and the specificity was determined in a group of 118 patients with other rheumatic diseases. The optimal cutoff value of the test was determined by ROC curve analysis. RESULTS: The frequency of ANA positivity in healthy subjects was 13.2%. There was no difference in the frequency of positive results according to gender or age. The abnormal title of ANA was defined as the dilution of 1:160. The 1:80 dilution had sensitivity of 87.7% and specificity of 67.8%, while the 1:160 dilution had sensitivity of 82% and specificity of 73.7%. By ROC curve analysis, a dilution of 1:160 corresponded to the optimal cutoff value. CONCLUSION: The abnormal title and the optimal cutoff value of ANA HEp-2 in the population was 1:160. Therefore, the dilution of 1:160 is the optimal screening dilution, with better specificity but without significantly compromising the sensitivity of the diagnostic test.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Autoimunes / Anticorpos Antinucleares / Doenças Reumáticas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En / Pt Revista: Rev Bras Reumatol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doenças Autoimunes / Anticorpos Antinucleares / Doenças Reumáticas Tipo de estudo: Diagnostic_studies / Prognostic_studies / Screening_studies Limite: Adult / Female / Humans / Male / Middle aged Idioma: En / Pt Revista: Rev Bras Reumatol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil