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Efficient production of transgenic melon via Agrobacterium-mediated transformation.
Bezirganoglu, I; Hwang, S Y; Shaw, J F; Fang, T J.
Afiliação
  • Bezirganoglu I; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, China.
  • Hwang SY; Department of Entomology, National Chung Hsing University, Taichung, Taiwan, China.
  • Shaw JF; Agricultural Biotechnology Research Center, Academia Sinica, Taipei, China.
  • Fang TJ; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, China tjfang@nchu.edu.tw.
Genet Mol Res ; 13(2): 3218-27, 2014 Apr 25.
Article em En | MEDLINE | ID: mdl-24841654
Oriental melon (Cucumis melo L. var. makuwa) is an important fruit for human consumption. However, this plant species is one of the most recalcitrant to genetic transformation. The lack of an efficient in vitro system limits the development of a reproducible genetic transformation protocol for Oriental melon. In this study, an efficient transgenic production method for Agrobacterium-mediated transformation using cotyledon explants of Oriental melon was developed. Cotyledon explants were pre-cultivated for two days in the dark, and the optimal conditions for transformation of melon were determined to be a bacteria concentration of OD600 0.6, inoculation for 30 min, and two days of co-cultivation. Transgenic melon plants were produced from kanamycin-resistant shoots. A total of 11 independent transgenic plants were regenerated with a transformation efficiency of 0.8% of the inoculated explants. The transgenic plants were phenotypically normal and fully fertile, which might be a consequence of the co-cultivation time.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transformação Genética / Plantas Geneticamente Modificadas / Agrobacterium tumefaciens / Cucurbitaceae Limite: Humans Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transformação Genética / Plantas Geneticamente Modificadas / Agrobacterium tumefaciens / Cucurbitaceae Limite: Humans Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil