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Evaluation of tetravalent and conserved synthetic peptides vaccines derived from Dengue virus Envelope domain I and II.
Rocha, Raissa Prado; Livonesi, Márcia Cristina; Fumagalli, Marcilio Jorge; Rodrigues, Naiara Ferreira; da Costa, Lauro César Felipe; Dos Santos, Michelle Cristina Silva Gomes; de Oliveira Rocha, Eliseu Soares; Kroon, Erna Geessien; Malaquias, Luiz Cosme Cotta; Coelho, Luiz Felipe Leomil.
Afiliação
  • Rocha RP; Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Minas Gerais, Brazil.
  • Livonesi MC; Departamento de Análises Clínicas e Toxicológicas, Faculdade de Ciências Farmacêuticas, Universidade Federal de Alfenas, Minas Gerais, Brazil.
  • Fumagalli MJ; Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Minas Gerais, Brazil.
  • Rodrigues NF; Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Minas Gerais, Brazil.
  • da Costa LC; Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Minas Gerais, Brazil.
  • Dos Santos MC; Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Minas Gerais, Brazil.
  • de Oliveira Rocha ES; Laboratório de Vírus, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Kroon EG; Laboratório de Vírus, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Minas Gerais, Brazil.
  • Malaquias LC; Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Minas Gerais, Brazil.
  • Coelho LF; Laboratório de Vacinas, Instituto de Ciências Biomédicas, Universidade Federal de Alfenas, Minas Gerais, Brazil. Electronic address: coelho@unifal-mg.edu.br.
Virus Res ; 188: 122-7, 2014 Aug 08.
Article em En | MEDLINE | ID: mdl-24768848
Dengue is a major worldwide public health problem, especially in the tropical and subtropical regions of the world. Primary infection with a single Dengue virus (DENV) serotype causes a mild, self-limiting febrile illness called dengue fever. However, a subset of patients experiencing a secondary infection with a different serotype progress to the severe form of the disease, called dengue hemorrhagic fever. In this study, the vaccine potential of three tetravalent and conserved synthetic peptides derived from DENV envelope domain I (named Pep01) and II (named Pep02 and Pep03) was evaluated. Human dengue IgM/IgG positive serum (n=16) showed reactivity against Pep01, Pep02 and Pep03 in different degrees. Mice immunization experiments showed that these peptides were able to induce a humoral response characterized by antibodies with low neutralizing activity. The spleen cells derived from mice immunized with the peptides showed a significant cytotoxic activity (only for Pep02 and Pep03), a high expression of IL-10 (P<0.01) and a reduced expression of TNF-α and IFN-gamma (P<0.001) compared to DENV-1 infected splenocytes. Thus these peptides, and specially the Pep03, can induce a humoral response characterized by antibodies with low neutralizing activities and probably a T cell response that could be beneficial to induce an effective immune response against all DENV serotypes and do not contributed to the immunopathogenesis. However, further studies in peptide sequence will be required to induce the production of neutralizing antibodies against all four DENV serotypes and also to improve immunogenicity of these peptides.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas do Envelope Viral / Vírus da Dengue / Vacinas contra Dengue Limite: Animals Idioma: En Revista: Virus Res Assunto da revista: VIROLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas do Envelope Viral / Vírus da Dengue / Vacinas contra Dengue Limite: Animals Idioma: En Revista: Virus Res Assunto da revista: VIROLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda