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Uptake and transfer of a Bt toxin by a Lepidoptera to its eggs and effects on its offspring.
Paula, Débora Pires; Andow, David A; Timbó, Renata Velozo; Sujii, Edison R; Pires, Carmen S S; Fontes, Eliana M G.
Afiliação
  • Paula DP; Department of Biological Control, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.
  • Andow DA; Department of Entomology, University of Minnesota, St. Paul, Minnesota, United States of America.
  • Timbó RV; Department of Molecular Biology, University of Brasilia, Brasília, DF, Brazil.
  • Sujii ER; Department of Biological Control, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.
  • Pires CS; Department of Biological Control, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.
  • Fontes EM; Department of Biological Control, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.
PLoS One ; 9(4): e95422, 2014.
Article em En | MEDLINE | ID: mdl-24747962
Research on non-target effects of transgenic crop plants has focused primarily on bitrophic, tritrophic and indirect effects of entomotoxins from Bacillus thuringiensis, but little work has considered intergenerational transfer of Cry proteins. This work reports a lepidopteran (Chlosyne lacinia) taking up a Bt entomotoxin when exposed to sublethal or low concentrations, transferring the entomotoxin to eggs, and having adverse effects on the first filial generation (F1) offspring. Two bioassays were conducted using a sublethal concentration of toxin (100.0 ng/µl Cry1Ac) for adults and a concentration equal to the LC10 (2.0 ng/µl Cry1Ac) for larvae. Cry1Ac is the most common entomotoxin expressed in Bt cotton in Brazil. In the adult diet bioassay there was no adverse effect on the parental generation (P0) adults, but the F1 larvae had higher mortality and longer development time compared to F1 larvae of parents that did not ingest Cry1Ac. For the 3rd instar larvae, there was no measurable effect on the P0 larvae, pupae and adults, but the F1 larvae had higher mortality and longer development time. Using chemiluminescent Western Blot, Cry1Ac was detected in F1 eggs laid by P0 butterflies from both bioassays. Our study indicates that, at least for this species and these experimental conditions, a ∼65 kDa insecticidal protein can be taken up and transferred to descendants where it can increase mortality and development time.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Ovos / Endotoxinas / Proteínas Hemolisinas / Lepidópteros Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Ovos / Endotoxinas / Proteínas Hemolisinas / Lepidópteros Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos