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Development and characterization of novel SSR markers in Siniperca kneri Garman.
Yang, M; Zheng, H Z; Liang, X-F; Tian, C X; Dou, Y Q; Zhu, K C; Yuan, Y C.
Afiliação
  • Yang M; Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
  • Zheng HZ; Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
  • Liang XF; Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
  • Tian CX; Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
  • Dou YQ; Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
  • Zhu KC; Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
  • Yuan YC; Key Lab of Freshwater Animal Breeding, College of Fisheries, Huazhong Agricultural University, Ministry of Agriculture, Wuhan, Hubei, China.
Genet Mol Res ; 13(3): 7593-606, 2014 Mar 24.
Article em En | MEDLINE | ID: mdl-24737510
In this study, 37 transcriptome-derived simple sequence repeat (SSR) markers and 18 genomic SSR markers were developed and characterized in the Chinese perch, Siniperca kneri Garman. The average allele number per locus was 5.1 (range: 2-8) for transcriptome-derived SSRs and 3.8 (range: 2-5) for genomic SSRs. The average observed and expected heterozygosities were 0.666 (range: 0.000-1.000) and 0.692 (range: 0.230-0.857) for transcriptome-derived SSRs, respectively. These values were 0.380 (range: 0.000-1.000) and 0.527 (range: 0.201-0.799) for genomic SSRs, respectively. The average polymorphic information content was 0.638 (range: 0.215-0.824) for transcriptome-derived SSRs and 0.477 (range: 0.183-0.752) for genomic SSRs. Seven of these loci exhibited departure from Hardy-Weinberg equilibrium after sequential Bonferroni's correction for multiple tests, and no significant deviation was observed for the linkage disequilibrium. These developed and characterized markers are anticipated to be useful for studies on population genetics, conservation genetics, and the fishery management of this species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Percas / Repetições de Microssatélites Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Percas / Repetições de Microssatélites Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil