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Methylobacterium-plant interaction genes regulated by plant exudate and quorum sensing molecules.
Dourado, Manuella Nóbrega; Bogas, Andrea Cristina; Pomini, Armando M; Andreote, Fernando Dini; Quecine, Maria Carolina; Marsaioli, Anita J; Araújo, Welington Luiz.
Afiliação
  • Dourado MN; Departamento de Genética, Universidade de São Paulo, Piracicaba, SP, Brazil.
  • Bogas AC; Departamento de Genética, Universidade de São Paulo, Piracicaba, SP, Brazil.
  • Pomini AM; Departamento de Química, Universidade Estadual de Maringá, Maringá, PR, Brazil.
  • Andreote FD; Departamento de Ciências do Solos, Escola Superior de Agricultura "Luiz de Queiróz", Universidade de São Paulo, Piracicaba, SP, Brazil.
  • Quecine MC; Departamento de Genética, Universidade de São Paulo, Piracicaba, SP, Brazil.
  • Marsaioli AJ; Instituto de Química, Universidade de Campinas, Campinas, São Paulo, Brazil.
  • Araújo WL; Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.
Braz J Microbiol ; 44(4): 1331-9, 2013 Dec.
Article em En | MEDLINE | ID: mdl-24688531
Bacteria from the genus Methylobacterium interact symbiotically (endophytically and epiphytically) with different plant species. These interactions can promote plant growth or induce systemic resistance, increasing plant fitness. The plant colonization is guided by molecular communication between bacteria-bacteria and bacteria-plants, where the bacteria recognize specific exuded compounds by other bacteria (e.g. homoserine molecules) and/or by the plant roots (e.g. flavonoids, ethanol and methanol), respectively. In this context, the aim of this study was to evaluate the effect of quorum sensing molecules (N-acyl-homoserine lactones) and plant exudates (including ethanol) in the expression of a series of bacterial genes involved in Methylobacterium-plant interaction. The selected genes are related to bacterial metabolism (mxaF), adaptation to stressful environment (crtI, phoU and sss), to interactions with plant metabolism compounds (acdS) and pathogenicity (patatin and phoU). Under in vitro conditions, our results showed the differential expression of some important genes related to metabolism, stress and pathogenesis, thereby AHL molecules up-regulate all tested genes, except phoU, while plant exudates induce only mxaF gene expression. In the presence of plant exudates there is a lower bacterial density (due the endophytic and epiphytic colonization), which produce less AHL, leading to down regulation of genes when compared to the control. Therefore, bacterial density, more than plant exudate, influences the expression of genes related to plant-bacteria interaction.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas / Extratos Vegetais / Regulação Bacteriana da Expressão Gênica / Methylobacterium / Acil-Butirolactonas / Interações Hospedeiro-Parasita Idioma: En Revista: Braz J Microbiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
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Imprimir
XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plantas / Extratos Vegetais / Regulação Bacteriana da Expressão Gênica / Methylobacterium / Acil-Butirolactonas / Interações Hospedeiro-Parasita Idioma: En Revista: Braz J Microbiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil