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Expression of the matrix metalloproteases 2, 14, 24, and 25 and tissue inhibitor 3 as potential molecular markers in advanced human gastric cancer.
de la Peña, Sol; Sampieri, Clara Luz; Ochoa-Lara, Mariana; León-Córdoba, Kenneth; Remes-Troche, José María.
Afiliação
  • de la Peña S; Biomedical Sciences Doctoral Program, Biomedical Research Center, University of Veracruz, 91190 Xalapa, Veracruz, Mexico.
  • Sampieri CL; Institute of Public Health, University of Veracruz, 91190 Xalapa, Veracruz, Mexico.
  • Ochoa-Lara M; Public Health Master Program, University of Veracruz, 91190 Xalapa, Veracruz, Mexico.
  • León-Córdoba K; Dr. Miguel Dorantes Mesa Hospital, 91130 Xalapa, Veracruz, Mexico.
  • Remes-Troche JM; Medical-Biological Research Institute, University of Veracruz, 91700 Veracruz, Veracruz, Mexico.
Dis Markers ; 2014: 285906, 2014.
Article em En | MEDLINE | ID: mdl-24669030
BACKGROUND: During progression of gastric cancer (GC), degradation of the extracellular matrix is mediated by the matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs): changes in the expression of these have been related to unfavorable prognosis in GC. OBJECTIVE: To analyze the expression of certain MMPs and TIMPs in chronic superficial gastritis (SG) and GC. METHODS: The expression of MMPs and TIMPs was determined using qRT-PCR; the expression was classified, using threshold cycle (C(T)) values, as very high (C(T) ≤ 25), high (C(T) = 26-30), moderate (C(T) = 31-35), low (C(T) = 36-39), or not detected (C(T) = 40). Strength of association was estimated between the proteins, which were detected by Western blot, and the risk of developing GC. RESULTS: We found a high expression of MMP1, MMP2, MMP14, TIMP1, and TIMP3; moderate one of MMP9 and MMP25, and low one of MMP13 and MMP24 in both tissues. In absolute mRNA levels, significant differences were found in expression of MMP2, MMP24, and MMP25, which are overexpressed in GC compared with SG. The presence of the proteins MMP-14 and TIMP-3 was associated with the risk of developing GC. CONCLUSIONS: We consider that MMP2, MMP24, and MMP25 and the proteins MMP-14 and TIMP-3 could be candidates for prognostic molecular markers in GC.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Biomarcadores Tumorais / Inibidor Tecidual de Metaloproteinase-3 / Metaloproteinases da Matriz Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Dis Markers Assunto da revista: BIOQUIMICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Gástricas / Biomarcadores Tumorais / Inibidor Tecidual de Metaloproteinase-3 / Metaloproteinases da Matriz Tipo de estudo: Observational_studies / Prognostic_studies / Risk_factors_studies Limite: Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Dis Markers Assunto da revista: BIOQUIMICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos