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Molecular cloning and immune response analysis of putative variable lipoproteins from Mycoplasma mycoides subsp capri.
Xu, C G; Hao, Y Q; Zhang, L; Hao, R X; Liu, X L; Huang, Z Y.
Afiliação
  • Xu CG; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Veterinary School, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
  • Hao YQ; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Veterinary School, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China haoyq1960@163.com.
  • Zhang L; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Veterinary School, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
  • Hao RX; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Veterinary School, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
  • Liu XL; Key Laboratory of Clinical Diagnosis and Treatment Techniques for Animal Disease, Ministry of Agriculture, Veterinary School, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
  • Huang ZY; Life Science & Chemistry School, Hulunbuir College, Hulunbuir, Inner Mongolia, China.
Genet Mol Res ; 13(1): 1527-39, 2014 Mar 12.
Article em En | MEDLINE | ID: mdl-24668627
Mycoplasma mycoides subsp capri is the cause of goat "MAKePS" (Mastitis, Arthritis, Keratoconjunctivitis, Pneumonia, Septicemia) syndrome. We identified three genes (GL_ 000459; 000461; 000462) as variable lipoprotein genes in the M. mycoides subsp capri str. PG3 genome by genomic information and comparative genomic analyses. To study the role of variable lipoproteins in M. mycoides subsp capri pathogenesis and evaluate the immunogenic and protective potentials of those proteins, we constructed the expression systems and expressed the mature peptide portion of the three proteins in E. coli. We also determined the titers and opsonophagocytosis activity of total IgG antibodies and the levels of Th1 and Th2 cytokines in sera, and we ran a lymphocyte proliferation assay in mice immunized with recombinant proteins His-tag-GL000459, His-tag-GL000461, and His-tag-GL000462. These three lipoproteins induced humoral and cellular immune responses in the immunized mice. Additionally, the whole blood opsonophagocitic in vitro assay demonstrated that the antibodies produced by the immunized groups can neutralize strain PG3; consequently, these three variable lipoproteins could be the major surface antigens in M. mycoides subsp capri str. PG3.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cabras / Imunidade Inata / Lipoproteínas / Antígenos de Superfície Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cabras / Imunidade Inata / Lipoproteínas / Antígenos de Superfície Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil