Recombinant vaccinia/Venezuelan equine encephalitis (VEE) virus expresses VEE structural proteins.
J Gen Virol
; 69 ( Pt 12): 3005-13, 1988 Dec.
Article
em En
| MEDLINE
| ID: mdl-2462013
cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7.5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vaccinia virus
/
Proteínas Virais
/
Proteínas Recombinantes de Fusão
/
Proteínas Recombinantes
/
Vírus da Encefalite Equina Venezuelana
País/Região como assunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
J Gen Virol
Ano de publicação:
1988
Tipo de documento:
Article
País de publicação:
Reino Unido