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The contribution of transient receptor potential ankyrin 1 (TRPA1) to the in vivo nociceptive effects of prostaglandin E2.
Dall'Acqua, Marcelo C; Bonet, Ivan J M; Zampronio, Aleksander R; Tambeli, Cláudia H; Parada, Carlos A; Fischer, Luana.
Afiliação
  • Dall'Acqua MC; Department of Physiology, Division of Biological Sciences, Federal University of Parana, Curitiba, Parana, Brazil.
  • Bonet IJ; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
  • Zampronio AR; Department of Pharmacology, Division of Biological Sciences, Federal University of Parana, Curitiba, Parana, Brazil.
  • Tambeli CH; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
  • Parada CA; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
  • Fischer L; Department of Physiology, Division of Biological Sciences, Federal University of Parana, Curitiba, Parana, Brazil. Electronic address: fischer@ufpr.br.
Life Sci ; 105(1-2): 7-13, 2014 Jun 06.
Article em En | MEDLINE | ID: mdl-24607781
AIMS: Although evidence suggest that TRPA1 mediates some effects of prostaglandins, it is not known whether TRPA1 contributes to the in vivo nociceptive effects of prostaglandin E2 (PGE2), a key mediator of inflammatory pain. MAIN METHODS: To address this issue, the effect of the pharmacological blockade of TRPA1 or of its gene silencing on the hyperalgesia induced in the rat paw by PGE2 or its downstream signaling molecules, protein kinase A (PKA) or protein kinase C-epsilon (PKCε), was evaluated. TRPA1 expression on dorsal root ganglia cells was assessed by western blot. KEY FINDINGS: The pharmacological blockade of local TRPA1 by its selective antagonist, HC 030031 decreased and reversed PGE2-induced hyperalgesia. The TRPA1 gene silencing induced by intrathecal pre-treatment with antisense oligodeoxynucleotide blocked PGE2-induced hyperalgesia and strongly reduced TRPA1 expression in dorsal root ganglia cells (L5 and L6). PGE2 injection into the hind paw did not significantly increase TRPA1 expression in dorsal root ganglia cells. Treatment with either HC 030031 or antisense oligodeoxynucleotide significantly decreased the hyperalgesia induced by PKA or PKCε. Since both kinases are the major components of PGE2-induced intracellular signal transduction, the modulation of TRPA1 function by PGE2 may be downstream PKA and PKC-epsilon. SIGNIFICANCE: These findings show that TRPA1 is essential to the in vivo nociceptive effects induced by one of the most important mediators of inflammatory pain, PGE2. This is one of the crucial findings necessary to support TRPA1 as a promising target for the development of future drugs to pain treatment and control.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dinoprostona / Canais de Cátion TRPC / Nociceptividade / Gânglios Espinais Limite: Animals Idioma: En Revista: Life Sci Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dinoprostona / Canais de Cátion TRPC / Nociceptividade / Gânglios Espinais Limite: Animals Idioma: En Revista: Life Sci Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda