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Immunogenicity and protective efficacy of yeast extracts containing rotavirus-like particles: a potential veterinary vaccine.
Rodríguez-Limas, William A; Pastor, Ana Ruth; Esquivel-Soto, Ernesto; Esquivel-Guadarrama, Fernando; Ramírez, Octavio T; Palomares, Laura A.
Afiliação
  • Rodríguez-Limas WA; Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico.
  • Pastor AR; Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico.
  • Esquivel-Soto E; Facultad de Medicina, Universidad Autónoma del Estado de Morelos, CP 62350 Cuernavaca, Morelos, Mexico.
  • Esquivel-Guadarrama F; Facultad de Medicina, Universidad Autónoma del Estado de Morelos, CP 62350 Cuernavaca, Morelos, Mexico.
  • Ramírez OT; Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico.
  • Palomares LA; Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, CP 62250 Cuernavaca, Morelos, Mexico. Electronic address: laura@ibt.unam.mx.
Vaccine ; 32(24): 2794-8, 2014 May 19.
Article em En | MEDLINE | ID: mdl-24593996
Rotavirus is the most common cause of severe diarrhea in many animal species of economic interest. A simple, safe and cost-effective vaccine is required for the control and prevention of rotavirus in animals. In this study, we evaluated the use of Saccharomyces cerevisiae extracts containing rotavirus-like particles (RLP) as a vaccine candidate in an adult mice model. Two doses of 1mg of yeast extract containing rotavirus proteins (between 0.3 and 3 µg) resulted in an immunological response capable of reducing the replication of rotavirus after infection. Viral shedding in all mice groups diminished in comparison with the control group when challenged with 100 50% diarrhea doses (DD50) of murine rotavirus strain EDIM. Interestingly, when immunizing intranasally protection against rotavirus infection was observed even when no increase in rotavirus-specific antibody titers was evident, suggesting that cellular responses were responsible of protection. Our results indicate that raw yeast extracts containing rotavirus proteins and RLP are a simple, cost-effective alternative for veterinary vaccines against rotavirus.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Saccharomyces cerevisiae / Vacinas contra Rotavirus / Vacinas de Partículas Semelhantes a Vírus Limite: Animals Idioma: En Revista: Vaccine Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Holanda
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Rotavirus / Saccharomyces cerevisiae / Vacinas contra Rotavirus / Vacinas de Partículas Semelhantes a Vírus Limite: Animals Idioma: En Revista: Vaccine Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Holanda