Your browser doesn't support javascript.
loading
Direct and indirect air particle cytotoxicity in human alveolar epithelial cells.
Orona, N S; Astort, F; Maglione, G A; Saldiva, P H N; Yakisich, J S; Tasat, D R.
Afiliação
  • Orona NS; School of Science and Technology, National University of General San Martín, Martín de Irigoyen 3100, 1653 San Martín, Buenos Aires, Argentina; Committee for Scientific Research, calle 526 entre 10 y 11, 1900 La Plata, Buenos Aires, Argentina.
  • Astort F; School of Science and Technology, National University of General San Martín, Martín de Irigoyen 3100, 1653 San Martín, Buenos Aires, Argentina.
  • Maglione GA; School of Science and Technology, National University of General San Martín, Martín de Irigoyen 3100, 1653 San Martín, Buenos Aires, Argentina.
  • Saldiva PH; Institute of Integrated Risk Analysis, National Research Council, Brazil.
  • Yakisich JS; Department of Clinical Neuroscience, Karolinska Institute, Stockholm, Sweden; Preclinical Research, LLC, Norfolk, VA 23510, USA.
  • Tasat DR; School of Science and Technology, National University of General San Martín, Martín de Irigoyen 3100, 1653 San Martín, Buenos Aires, Argentina; School of Dentistry, University of Buenos Aires, MT de Alvear 2142, C1122AAH Buenos Aires, Argentina. Electronic address: dtasat@unsam.edu.ar.
Toxicol In Vitro ; 28(5): 796-802, 2014 Aug.
Article em En | MEDLINE | ID: mdl-24590061
Air particulate matter has been associated with adverse impact on the respiratory system leading to cytotoxic and proinflammatory effects. The biological mechanisms behind these associations may be initiated by inhaled small size particles, particle components (soluble fraction) and/or mediators released by particle-exposed cells (conditioned media). The effect of Urban Air Particles from Buenos Aires (UAP-BA) and Residual Oil Fly Ash (ROFA) a surrogate of ambient air pollution, their Soluble Fractions (SF) and Conditioned Media (CM) on A549 lung epithelial cells was examined. After 24 h exposure to TP (10 and 100 µg/ml), SF or CM, several biological parameters were assayed on cultured A549 cells. We tested cell viability by MTT, superoxide anion (O2(-)) generation by NBT and proinflammatory cytokine (TNFα, IL-6 and IL-8) production by ELISA. UAP-BA particles or its SF (direct effect) did not modify cell viability and generation of O2(-) for any of the doses tested. On the contrary, UAP-BA CM (indirect effect) reduced cell viability and increased both generation of O2(-) and IL-8 production. Exposure to ROFA particles, SF or ROFA CM reduced proliferation and O2(-) but, stimulated IL-8. It is worth to note that UAP-BA and ROFA depicted distinct effects on particle-exposed A549 cells implicating morphochemical dependence. These in vitro findings support the hypothesis that particle-induced lung inflammation and disease may involve lung-derived mediators.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Alvéolos Pulmonares / Poluentes Atmosféricos / Células Epiteliais / Material Particulado Limite: Humans País/Região como assunto: America do sul / Argentina Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Alvéolos Pulmonares / Poluentes Atmosféricos / Células Epiteliais / Material Particulado Limite: Humans País/Região como assunto: America do sul / Argentina Idioma: En Revista: Toxicol In Vitro Assunto da revista: TOXICOLOGIA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido