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Comparison of extraction methods of chitin from Ganoderma lucidum mushroom obtained in submerged culture.
Ospina Álvarez, Sandra Patricia; Ramírez Cadavid, David Alexander; Escobar Sierra, Diana Marcela; Ossa Orozco, Claudia Patricia; Rojas Vahos, Diego Fernando; Zapata Ocampo, Paola; Atehortúa, Lucía.
Afiliação
  • Ospina Álvarez SP; Biotechnology Group, Natural Sciences Faculty, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia.
  • Ramírez Cadavid DA; Biotechnology Group, Natural Sciences Faculty, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia.
  • Escobar Sierra DM; Biomaterials and Biomechanical Research Group, Bioengineering Program, Universidad de Antioquia, Calle 67 No. 53-108, Bloque 18-227 Medellín, Colombia.
  • Ossa Orozco CP; Biomaterials and Biomechanical Research Group, Bioengineering Program, Universidad de Antioquia, Calle 67 No. 53-108, Bloque 18-227 Medellín, Colombia.
  • Rojas Vahos DF; Biotechnology Group, Natural Sciences Faculty, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia.
  • Zapata Ocampo P; Biotechnology Group, Natural Sciences Faculty, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia.
  • Atehortúa L; Biotechnology Group, Natural Sciences Faculty, Universidad de Antioquia, Calle 70 No. 52-21, Medellín, Antioquia, Colombia.
Biomed Res Int ; 2014: 169071, 2014.
Article em En | MEDLINE | ID: mdl-24551839
The chitin was isolated from the Ganoderma lucidum submerged cultures mycelium as potential source of chitin under biotechnological processes. The extraction of chitin was carried out through 5 different assays which involved mainly three phases: pulverization of the mushroom, deproteinization of the mycelia with NaOH solution, and a process of decolorization with potassium permanganate and oxalic acid. The chitin contents extracted from 9-day mycelia were 413, 339, 87, 78, and 144 mg/g(-1) (milligrams of chitin/grams of dry biomass) for A1, A2, A3, A4, and A5, respectively. Obtained chitin was characterized by X-Ray Diffraction (XRD), by Fourier transform infrared spectroscopy (FTIR), and by thermal analysis (TGA). The results showed that Ganoderma lucidum chitin has similar characteristic of chitin from different fonts. The advantage of the biotechnological processes and the fact that Ganoderma lucidum fungus may be used as a potential raw material for chitin production were demonstrated.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biotecnologia / Quitina / Técnicas de Cultura de Células Idioma: En Revista: Biomed Res Int Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Biotecnologia / Quitina / Técnicas de Cultura de Células Idioma: En Revista: Biomed Res Int Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Colômbia País de publicação: Estados Unidos