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Alcoholic and isocaloric diet, but not ovariectomy, influence the apoptosis of bone cells within the alveolar bone crest of rats.
Marchini, Adriana Mathias Pereira da Silva; Gonçalves, Lucélia Lemes; Salgado, Miriane Carneiro Machado; do Prado, Renata Falchete; Marchini, Leonardo; Carvalho, Yasmin Rodarte; da Rocha, Rosilene Fernandes.
Afiliação
  • Marchini AM; Department of Biosciences and Diagnostics, Institute of Science and Technology, UNESP - State University of Sao Paulo, São José dos Campos, São Paulo, Brazil.
  • Gonçalves LL; Restorative Dentistry Department, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos, São Paulo, Brazil.
  • Salgado MC; Department of Biosciences and Diagnostics, Institute of Science and Technology, UNESP - State University of Sao Paulo, São José dos Campos, São Paulo, Brazil.
  • do Prado RF; Department of Biosciences and Diagnostics, Institute of Science and Technology, UNESP - State University of Sao Paulo, São José dos Campos, São Paulo, Brazil.
  • Marchini L; Department of Preventive and Community Dentistry, College of Dentistry, University of Iowa, Iowa City, IA, USA. Electronic address: leonardo-marchini@uiowa.edu.
  • Carvalho YR; Department of Biosciences and Diagnostics, Institute of Science and Technology, UNESP - State University of Sao Paulo, São José dos Campos, São Paulo, Brazil.
  • da Rocha RF; Department of Biosciences and Diagnostics, Institute of Science and Technology, UNESP - State University of Sao Paulo, São José dos Campos, São Paulo, Brazil.
Arch Oral Biol ; 59(4): 424-33, 2014 Apr.
Article em En | MEDLINE | ID: mdl-24549089
OBJECTIVE: Studies suggest that chronic alcoholism as well as oestrogen deficiencies may affect bones in general, including alveolar bone and, by doing so, increase individuals' susceptibility to develop progressive periodontal disease. This paper aims to verify the influence of chronic alcoholism and/or oestrogen deficiencies in the apoptosis of bone cells of the alveolar bone crest region in rats. DESIGN: Initially, 54 rats were divided into ovariectomized (Ovx) and Sham operated (Sham) groups. Thirty days after surgery, these two groups were equally sub-divided, and received, for 56 days, the following dietary intervention: alcoholic diet (with 20% alcohol solution,), isocaloric diet and ad libitum diet (free diet). Analysis was undertaken by immunohistochemistry, using an antibody to detect apoptosis (anti PARP p-85). RESULTS: When comparing the six experimental groups, no significant differences were observed in the apoptosis of bone cells. Also, there was no significant difference in the quantity of cells undergoing apoptosis when the animals from Ovx groups were compared with those from Sham groups. However, when comparing only different dietary groups, differences were observed between the groups ad libitum and isocaloric, to osteoblasts (p=0.045); and ad libitum and alcohol, to osteocytes (p=0.007). CONCLUSION: It is concluded that ovariectomy was not able to influence the rate of apoptosis of bone cells of the alveolar bone crest region in rats and that a possible influence of diet on apoptosis of osteoblasts and osteocytes cannot be ruled out.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Ovariectomia / Apoptose / Alcoolismo / Processo Alveolar Limite: Animals Idioma: En Revista: Arch Oral Biol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Ovariectomia / Apoptose / Alcoolismo / Processo Alveolar Limite: Animals Idioma: En Revista: Arch Oral Biol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido