Molecular characteristics and cloning of two pepper genes AN2 and UPA20.

Chen, H X; Jiang, H; Huo, J L; Wen, J F; Zhu, H S; Ma, C H; Zhou, H; Lv, J H; Deng, M H

Chen, H X; Jiang, H; Huo, J L; Wen, J F; Zhu, H S; Ma, C H; Zhou, H; Lv, J H; Deng, M H.

Afiliação

Chen HX; College of Horticulture, Hunan Agricultural University, Changsha, China.
Jiang H; College of Horticulture, Hunan Agricultural University, Changsha, China.
Huo JL; Yunnan Agricultural University, Kunming, China.
Wen JF; Kunming University of Science and Technology, Kunming, China wenjf888@163.com.
Zhu HS; Yunnan Agricultural University, Kunming, China.
Ma CH; Yunnan Agricultural University, Kunming, China.
Zhou H; Yunnan Agricultural University, Kunming, China.
Lv JH; Yunnan Agricultural University, Kunming, China.
Deng MH; Yunnan Agricultural University, Kunming, China.

Genet Mol Res ; 13(2): 2531-8, 2014 Jan 17.

Article em En | MEDLINE | ID: mdl-24535901

RESUMO

The complete coding sequences (CDSs) of "Yunnan Purple Pepper No.1" (Capsicum annuum L.) AN2 and UPA20 genes were amplified using the reverse transcriptase polymerase chain reaction on the basis of the conserved sequence information of some Solanaceae plants and known highly homologous pepper expressed sequence tags. The nucleotide sequence analysis of these 2 genes revealed that pepper AN2 gene encoded a protein of 263 amino acids that has high homology with the AN2-like protein of 4 species: tobacco, tomato, potato, and petunia. The UPA20 gene encoded a protein of 341 amino acids that has high homology with the proteins of 3 species: tobacco, petunia, and tomato. The tissue expression analysis indicated that the pepper AN2 gene was overexpressed in the pericarp and placenta; moderately in stems, flowers, and seeds; and weakly in the roots, leaves, and pericarp. The pepper UPA20 gene was overexpressed in the flowers and seeds; moderately expressed in the roots and stems; and weakly expressed in the leaves and placenta. Our findings might form the basis for further research on these 2 pepper genes.

Assuntos

Capsicum/genética; Regulação da Expressão Gênica de Plantas; Proteínas de Plantas/biossíntese; China; Clonagem Molecular; Flores/genética; Flores/metabolismo; Folhas de Planta/genética; Folhas de Planta/metabolismo; Proteínas de Plantas/genética; Análise de Sequência de DNA; Distribuição Tecidual

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Capsicum / Regulação da Expressão Gênica de Plantas País/Região como assunto: Asia Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil

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