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Differential contribution of BDNF and NGF to long-term potentiation in the superior cervical ganglion of the rat.
Arias, Erwin R; Valle-Leija, Pablo; Morales, Miguel A; Cifuentes, Fredy.
Afiliação
  • Arias ER; Departamento de Biología Celular & Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 3er Circuito Exterior s/número, Cd. Universitaria, México, D.F. 04510, Mexico.
  • Valle-Leija P; Departamento de Biología Celular & Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 3er Circuito Exterior s/número, Cd. Universitaria, México, D.F. 04510, Mexico.
  • Morales MA; Departamento de Biología Celular & Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 3er Circuito Exterior s/número, Cd. Universitaria, México, D.F. 04510, Mexico.
  • Cifuentes F; Departamento de Biología Celular & Fisiología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 3er Circuito Exterior s/número, Cd. Universitaria, México, D.F. 04510, Mexico. Electronic address: fcifuent@biomedicas.unam.mx.
Neuropharmacology ; 81: 206-14, 2014 Jun.
Article em En | MEDLINE | ID: mdl-24530966
Synaptic transmission in the sympathetic nervous system is a plastic process modulated by different factors. We characterized the effects of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) on basal transmission and ganglionic long-term potentiation (LTP) in the rat superior cervical ganglion. LTP was elicited by supramaximal tetanic stimulation (40 Hz, 3 s) of the sympathetic trunk and was quantified by measuring LTP decay time and LTP extent. Neurotrophins did not affect basal transmission, however, they differentially affected LTP. BDNF (200 ng/ml) increased LTP decay time and LTP extent 2.0-fold (p < 0.01). In contrast, NGF showed a dual effect: 200 ng/ml NGF reduced LTP decay time and LTP extent to 53% and to 32% of control value (p < 0.0001 and p < 0.02; respectively), whereas >350 ng/ml NGF significantly increased LTP decay time and LTP extent (p < 0.02). Digital analysis of compound action potentials suggests that neurotrophins could change the synchronization of unitary action potentials. Pharmacological data obtained in intact ganglia show that C2-ceramide produced a 2-fold enhancement in LTP, whereas tyrphostin AG879, an inhibitor of tyrosine kinase activity, reversed the NGF blockade and produced by itself an enhancement in LTP. In sliced ganglia we observed that an anti-TrkA antibody reversed the NGF-induced LTP blockade. Immunohistochemistry studies revealed that 83% of ganglionic neurons express TrkA, whereas 52% express p75 receptor, and 18% express TrkB receptor. We propose that p75 neurotrophin receptors and probably TrkB signaling enhance LTP, whereas TrkA signaling reduces it.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gânglio Cervical Superior / Potenciação de Longa Duração / Fator Neurotrófico Derivado do Encéfalo / Fator de Crescimento Neural / Neurônios Limite: Animals Idioma: En Revista: Neuropharmacology Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Gânglio Cervical Superior / Potenciação de Longa Duração / Fator Neurotrófico Derivado do Encéfalo / Fator de Crescimento Neural / Neurônios Limite: Animals Idioma: En Revista: Neuropharmacology Ano de publicação: 2014 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido