Transcriptional regulation of the protein kinase A subunits in Saccharomyces cerevisiae: autoregulatory role of the kinase A activity.
Biochim Biophys Acta
; 1839(4): 275-87, 2014.
Article
em En
| MEDLINE
| ID: mdl-24530423
Protein kinase A (PKA) is a broad specificity protein kinase that controls a physiological response following the increment of cAMP as a consequence of a particular stimulus. The specificity of cAMP-signal transduction is maintained by several levels of control acting all together. Herein we present the study of the regulation of the expression of each PKA subunit, analyzing the activity of their promoters. The promoter of each isoform of TPK and of BCY1 is differentially activated during the growth phase. A negative mechanism of isoform-dependent autoregulation directs TPKs and BCY1 gene expressions. TPK1 promoter activity is positively regulated during heat shock and saline stress. The kinase Rim15, but not the kinase Yak1, positively regulates TPK1 promoter. Msn2/4, Gis1, and Sok2 are transcription factors involved in the regulation of TPK1 expression during stress. TPK2, TPK3, and BCY1 promoters, unlike TPK1, are not activated under stress conditions, although all the promoters are activated under low or null protein kinase A activity. These results indicate that subunits share an inhibitory autoregulatory mechanism but have different mechanisms involved in response to heat shock or saline stress.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Regulação Fúngica da Expressão Gênica
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Proteínas Quinases Dependentes de AMP Cíclico
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Proteínas de Saccharomyces cerevisiae
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Subunidades Catalíticas da Proteína Quinase Dependente de AMP Cíclico
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Holanda