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Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee.
Tajabadi, Naser; Mardan, Makhdzir; Saari, Nazamid; Mustafa, Shuhaimi; Bahreini, Rasoul; Manap, Mohd Yazid Abdul.
Afiliação
  • Tajabadi N; Department of Food Sciences, Faculty of Food Sciences and Technology, University Putra Malaysia, Serdang, Selangor, Malaysia. ; Department of honey bee, Animal Science Research Institute of Iran, Karaj, Iran.
  • Mardan M; Department of Agriculture Technology, Faculty of Agriculture, University Putra Malaysia, Serdang, Selangor, Malaysia.
  • Saari N; Department of Food Sciences, Faculty of Food Sciences and Technology, University Putra Malaysia, Serdang, Selangor, Malaysia.
  • Mustafa S; Departments of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Serdang, Selangor, Malaysia.
  • Bahreini R; Department of honey bee, Animal Science Research Institute of Iran, Karaj, Iran.
  • Manap MY; Department of Food Technology, Faculty of Food Sciences and Technology, University Putra Malaysia, Serdang, Selangor, Malaysia.
Braz J Microbiol ; 44(3): 717-22, 2013.
Article em En | MEDLINE | ID: mdl-24516438
This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu".
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Abelhas / Lactobacillus Tipo de estudo: Diagnostic_studies Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Braz J Microbiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Abelhas / Lactobacillus Tipo de estudo: Diagnostic_studies Limite: Animals País/Região como assunto: Asia Idioma: En Revista: Braz J Microbiol Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Irã País de publicação: Brasil