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Efficient identification of ornamental peach cultivars using RAPD markers with a manual cultivar identification diagram strategy.
Han, J; Wang, W Y; Leng, X P; Guo, L; Yu, M L; Jiang, W B; Ma, R J.
Afiliação
  • Han J; College of Horticulture, Nanjing Agricultural University, Nanjing, China.
  • Wang WY; College of Horticulture, Nanjing Agricultural University, Nanjing, China.
  • Leng XP; College of Horticulture, Nanjing Agricultural University, Nanjing, China.
  • Guo L; Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
  • Yu ML; Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, China fangjingui@hotmail.com.
  • Jiang WB; College of Horticulture, Nanjing Agricultural University, Nanjing, China.
  • Ma RJ; Institute of Horticulture, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Genet Mol Res ; 13(1): 32-42, 2014 Jan 08.
Article em En | MEDLINE | ID: mdl-24446285
One of the most important uses of DNA markers is cultivar identification. However, no DNA fingerprint analysis strategy is available for making DNA markers helpful in practical plant cultivar identification, especially for the identification of a large number of cultivars. We developed a manual cultivar identification diagram strategy for efficient identification of plant cultivars, from which a cultivar identification diagram (CID) of genotyped plant individuals can be constructed manually. This CID could be used as a reference for quick identification of plant cultivars of interest. We used 11-mer RAPD primers to amplify DNA samples of 32 ornamental peach genotypes; all the cultivars were well distinguished by fingerprints from 6 primers. The utility of this CID was verified by identification of three randomly chosen groups of cultivars among the 32 ones that we selected. This CID generated will be useful for the identification of commercially important ornamental peach cultivars.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnica de Amplificação ao Acaso de DNA Polimórfico / Prunus Tipo de estudo: Diagnostic_studies / Guideline Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnica de Amplificação ao Acaso de DNA Polimórfico / Prunus Tipo de estudo: Diagnostic_studies / Guideline Idioma: En Revista: Genet Mol Res Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: China País de publicação: Brasil