High passage numbers induce resistance to apoptosis in C2C12 muscle cells.
Biocell
; 37(1): 1-9, 2013 Apr.
Article
em En
| MEDLINE
| ID: mdl-24396996
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (< 20) or high (> 60) passage numbers (identified as 1-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2Cl2 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxidantes
/
Apoptose
/
Mioblastos Esqueléticos
/
Peróxido de Hidrogênio
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Revista:
Biocell
Assunto da revista:
BIOLOGIA MOLECULAR
/
DIAGNOSTICO POR IMAGEM
/
HISTOCITOQUIMICA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Argentina
País de publicação:
Estados Unidos