Hantavirus Gn and Gc glycoproteins self-assemble into virus-like particles.

Acuña, Rodrigo; Cifuentes-Muñoz, Nicolás; Márquez, Chantal L; Bulling, Manuela; Klingström, Jonas; Mancini, Roberta; Lozach, Pierre-Yves; Tischler, Nicole D

Acuña, Rodrigo; Cifuentes-Muñoz, Nicolás; Márquez, Chantal L; Bulling, Manuela; Klingström, Jonas; Mancini, Roberta; Lozach, Pierre-Yves; Tischler, Nicole D.

Afiliação

Acuña R; Molecular Virology Laboratory, Fundación Ciencia & Vida, Santiago, Chile.

J Virol ; 88(4): 2344-8, 2014 Feb.

Article em En | MEDLINE | ID: mdl-24335294

RESUMO

How hantaviruses assemble and exit infected cells remains largely unknown. Here, we show that the expression of Andes (ANDV) and Puumala (PUUV) hantavirus Gn and Gc envelope glycoproteins lead to their self-assembly into virus-like particles (VLPs) which were released to cell supernatants. The viral nucleoprotein was not required for particle formation. Further, a Gc endodomain deletion mutant did not abrogate VLP formation. The VLPs were pleomorphic, exposed protrusions and reacted with patient sera.

Assuntos

Orthohantavírus/metabolismo; Virus Puumala/metabolismo; Proteínas do Envelope Viral/metabolismo; Vírion/metabolismo; Western Blotting; Reações Cruzadas/imunologia; Primers do DNA/genética; Ensaio de Imunoadsorção Enzimática; Orthohantavírus/genética; Humanos; Virus Puumala/genética; Vírion/genética

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírion / Proteínas do Envelope Viral / Orthohantavírus / Virus Puumala Limite: Humans Idioma: En Revista: J Virol Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos

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