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Aberrant telomerase expression in the endometrium of infertile women with deep endometriosis.
Mafra, Fernanda A; Christofolini, Denise M; Cavalcanti, Viviane; Vilarino, Fabia L; André, Gustavo M; Kato, Patricia; Bianco, Bianca; Barbosa, Caio P.
Afiliação
  • Mafra FA; Ideia Fértil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André/SP, Brazil.
  • Christofolini DM; Ideia Fértil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André/SP, Brazil.
  • Cavalcanti V; Ideia Fértil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André/SP, Brazil.
  • Vilarino FL; Ideia Fértil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André/SP, Brazil.
  • André GM; Ideia Fértil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André/SP, Brazil.
  • Kato P; Technical Support, Applied Biosystems, São Paulo/SP, Brazil.
  • Bianco B; Ideia Fértil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André/SP, Brazil. Electronic address: bianca.bianco@hotmail.com.
  • Barbosa CP; Ideia Fértil Institute, Human Reproduction and Genetics Center, Faculdade de Medicina do ABC, Santo André/SP, Brazil.
Arch Med Res ; 45(1): 31-5, 2014 Jan.
Article em En | MEDLINE | ID: mdl-24321595
BACKGROUND AND AIMS: Considering the complex cellular and molecular mechanisms involved in endometriosis formation and progression and the similarities concerning the association of endometriosis with tumorigenesis and metastasis, we hypothesized a possible relationship between telomerase and the development/progression of endometriosis. The present study aimed to evaluate the expression of telomerase in the endometrium and peritoneal endometriotic lesions from women with endometriosis and controls. METHODS: A case-control study was performed comprising 25 infertile women with endometriosis and 44 fertile women without endometriosis as controls. Samples of endometrium and endometriotic peritoneal lesions of the same patient were harvested in the late luteal phase of the cycle. The expression of hTERT and GAPDH genes was measured by mRNA using qRT-PCR based on TaqMan methodology. Student t test was used to compare the values between the groups; p >0.05 was accepted as statistically significant. RESULTS: The mean expression of hTERT in the endometriosis group was significantly high when compared to the control group (1.24 ± 4.67 vs. 0.31 ± 1.10, p = 0.026). When the expression of hTERT was compared in relation to disease stage, the group of moderate/severe endometriosis showed increased expression in relation to control group (2.59 ± 7.35 vs. 0.31 ± 1.10, p = 0.026). Regarding endometriotic peritoneal lesions, only one 1/25 expressed hTERT mRNA. This patient had deep endometriosis. CONCLUSIONS: There was an association between the expression of telomerase (hTERT mRNA) and the genesis and progression of endometriosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Telomerase / Endometriose / Endométrio / Infertilidade Feminina Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Female / Humans Idioma: En Revista: Arch Med Res Assunto da revista: MEDICINA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Telomerase / Endometriose / Endométrio / Infertilidade Feminina Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Adult / Female / Humans Idioma: En Revista: Arch Med Res Assunto da revista: MEDICINA Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos