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Proteins related to the spindle and checkpoint mitotic emphasize the different pathogenesis of hypoplastic MDS.
Heredia, Fabiola Fernandes; de Sousa, Juliana Cordeiro; Ribeiro Junior, Howard Lopes; Carvalho, Alex Fiorini; Magalhaes, Silvia Maria Meira; Pinheiro, Ronald Feitosa.
Afiliação
  • Heredia FF; Cancer Institute of Ceará/A.C. Camargo Hospital (Dinter-Minter), Fortaleza, Ceará, Brazil.
  • de Sousa JC; Federal University of Ceará, Brazil.
  • Ribeiro Junior HL; Federal University of Ceará, Brazil.
  • Carvalho AF; A.C. Camargo Hospital, São Paulo, Brazil.
  • Magalhaes SM; Department of Clinical Medicine, Division of Hematology, Walter Cantidio Hospital, Federal University of Ceará, Brazil.
  • Pinheiro RF; Department of Clinical Medicine, Division of Hematology, Walter Cantidio Hospital, Federal University of Ceará, Brazil. Electronic address: ronaldpinheiro@pq.cnpq.br.
Leuk Res ; 38(2): 218-24, 2014 Feb.
Article em En | MEDLINE | ID: mdl-24314588
Some studies show that alterations in expression of proteins related to mitotic spindle (AURORAS KINASE A and B) and mitotic checkpoint (CDC20 and MAD2L1) are involved in chromosomal instability and tumor progression in various solid and hematologic malignancies. This study aimed to evaluate these genes in MDS patients. The cytogenetics analysis was carried out by G-banding, AURKA and AURKB amplification was performed using FISH, and AURKA, AURKB, CDC20 and MAD2L1 gene expression was performed by qRT-PCR in 61 samples of bone marrow from MDS patients. AURKA gene amplification was observed in 10% of the cases, which also showed higher expression levels than the control group (p=0.038). Patients with normo/hypercellular BM presented significantly higher expression levels than hypocellular BM patients, but normo and hypercellular BM groups did not differ. After logistic regression analysis, our results showed that HIGH expression levels were associated with increased risk of developing normo/hypercellular MDS. It also indicated that age is associated with AURKA, CDC20 and MAD2L1 HIGH expression levels. The distinct expression of hypocellular patients emphasizes the prognostic importance of cellularity to MDS. The amplification/high expression of AURKA suggests that the increased expression of this gene may be related to the pathogenesis of disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Síndromes Mielodisplásicas / Proteínas de Ciclo Celular / Pontos de Checagem do Ciclo Celular / Fuso Acromático Tipo de estudo: Etiology_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Leuk Res Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Síndromes Mielodisplásicas / Proteínas de Ciclo Celular / Pontos de Checagem do Ciclo Celular / Fuso Acromático Tipo de estudo: Etiology_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: Leuk Res Ano de publicação: 2014 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido