Comparison of three decontamination methods for Mycobacterium bovis isolation.
Braz J Microbiol
; 39(2): 241-4, 2008 Apr.
Article
em En
| MEDLINE
| ID: mdl-24031209
SIXTY SAMPLES OF TISSUE FRAGMENTS WITH LESIONS SUGGESTIVE OF TUBERCULOSIS FROM BOVINE ABATTOIRS, KEPT IN SATURATED SOLUTION OF SODIUM BORATE, WERE SUBJECTED TO FOUR TREATMENTS: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Braz J Microbiol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil