Comparison of three decontamination methods for Mycobacterium bovis isolation.

Ambrosio, Simone Rodrigues; de Deus Oliveira, Eugenia Márcia; Rodriguez, Cesar Alejandro Rosales; Ferreira Neto, José Soares; Amaku, Marcos

Ambrosio, Simone Rodrigues; de Deus Oliveira, Eugenia Márcia; Rodriguez, Cesar Alejandro Rosales; Ferreira Neto, José Soares; Amaku, Marcos.

Afiliação

Ambrosio SR; Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia, Universidãde de São Paulo , São Paulo, SP , Brasil.

Braz J Microbiol ; 39(2): 241-4, 2008 Apr.

Article em En | MEDLINE | ID: mdl-24031209

RESUMO

SIXTY SAMPLES OF TISSUE FRAGMENTS WITH LESIONS SUGGESTIVE OF TUBERCULOSIS FROM BOVINE ABATTOIRS, KEPT IN SATURATED SOLUTION OF SODIUM BORATE, WERE SUBJECTED TO FOUR TREATMENTS: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.

Palavras-chave

HPC; Mycobacterium bovis; Petroff; decontamination

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Braz J Microbiol Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

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