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Ultrasonication and steam-explosion as chitin pretreatments for chitin oligosaccharide production by chitinases of Lecanicillium lecanii.
Villa-Lerma, Guadalupe; González-Márquez, Humberto; Gimeno, Miquel; López-Luna, Alberto; Bárzana, Eduardo; Shirai, Keiko.
Afiliação
  • Villa-Lerma G; Universidad Autonoma Metropolitana, Departamento de Biotecnologia, Laboratorio de Biopolimeros, San Rafael Atlixco 186, Mexico City C.P. 09340, Mexico.
  • González-Márquez H; Universidad Autonoma Metropolitana, Departamento de Biotecnologia, Laboratorio de Biopolimeros, San Rafael Atlixco 186, Mexico City C.P. 09340, Mexico.
  • Gimeno M; Universidad Nacional Autonoma de Mexico, Facultad de Quimica, Mexico City C.P. 04510, Mexico.
  • López-Luna A; Universidad Autonoma Metropolitana, Departamento de Biotecnologia, Laboratorio de Biopolimeros, San Rafael Atlixco 186, Mexico City C.P. 09340, Mexico.
  • Bárzana E; Universidad Nacional Autonoma de Mexico, Facultad de Quimica, Mexico City C.P. 04510, Mexico.
  • Shirai K; Universidad Autonoma Metropolitana, Departamento de Biotecnologia, Laboratorio de Biopolimeros, San Rafael Atlixco 186, Mexico City C.P. 09340, Mexico. Electronic address: smk@xanum.uam.mx.
Bioresour Technol ; 146: 794-798, 2013 Oct.
Article em En | MEDLINE | ID: mdl-23993287
In this study, chitin oligosaccharides have been successfully produced using chitinases from submerged fermentation of Lecanicillium lecanii. The highest Hex, Chit and Prot production was 0.14, 0.26 and 2.05 U/mg of protein, respectively, which were attained varying pH from 5 to 8 after 96 h. Culture conditions conducted at constant pH of 6 resulted in significantly lower enzyme production. The crude enzyme was partially purified by salting out with (NH4)2SO4 followed by size exclusion chromatography to isolate the chitinase mixture for further chitin hydrolysis assays. In this regard, chitin substrates were pretreated with sonication and steam explosion prior to enzymatic reaction. Structural changes were observed with steam explosion with 11.28% reduction of the crystallinity index attained with the lowest chitin/water ratio (0.1g/mL). Pretreated chitins reached the highest production of reducing sugars (0.37 mg/mL) and GlcNAc (0.59 mg/mL) in 23.6% yield.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Quitina / Quitinases / Quitosana / Hypocreales Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oligossacarídeos / Quitina / Quitinases / Quitosana / Hypocreales Idioma: En Revista: Bioresour Technol Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido