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Morphometric evaluation of the spermatogonial stem cell distribution and niche in vertebrates.
Campos-Junior, Paulo Henrique Almeida; Costa, Guilherme Mattos Jardim; de Avelar, Gleide Fernandes; Segatelli, Tânia Mara; Lacerda, Samyra Maria Santos Nassif; Aponte, Pedro Manuel; de França, Luiz Renato.
Afiliação
  • Campos-Junior PH; Laboratory of Cellular Biology, Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Methods Mol Biol ; 1035: 35-42, 2013.
Article em En | MEDLINE | ID: mdl-23959980
Morphometry is a classical quantitative method often used in biology to provide a data basis for functional interpretations/interactions of a particular organ or system. Herein we took advantage of this valuable approach to evaluate the spermatogonial stem cell niche using the horse testis and immunocytochemical localization of GFRA1 [glial cell line-derived neurotrophic factor receptor produced by Sertoli cells)] as an example. Using the NIH ImageJ free software, we describe in detail all the necessary steps to investigate this specific and crucial microenvironment. Based on several recently published papers from our research group, this approach has proved to be fast, simple, and adaptable to a wide range of species and has the potential to be easily reproducible in different laboratories.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Software / Células-Tronco Adultas / Nicho de Células-Tronco Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Software / Células-Tronco Adultas / Nicho de Células-Tronco Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos