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Evaluation of three sampling methods for the microbiological analysis of broiler carcasses after immersion chilling.
Giombelli, Audecir; Cavani, Ricardo; Gloria, Maria Beatriz Abreu.
Afiliação
  • Giombelli A; Laboratório de Bioquímica de Alimentos-LbqA, Faculdade de Farmácia, UFMG, Avenida Antonio Carlos 6627, Belo Horizonte, MG 31270-901, Brasil.
J Food Prot ; 76(8): 1330-5, 2013 Aug.
Article em En | MEDLINE | ID: mdl-23905787
Countries have different official programs and implement different sampling methods for the detection of Salmonella on poultry carcasses. In Brazil, a 25-g sample of skin and muscle excision (SME) from the wings, neck, and pericloacal parts is used; in the European Union (EU), a 25-g sample of neck skin (NSE) is used; and, in the United States, the whole carcass is rinsed with 400 ml of diluent (WCR). In the present study, these methods were evaluated to compare Salmonella occurrence and counts of hygiene indicator microorganisms (Escherichia coli, Enterobacteriaceae, and total viable count of aerobic mesophilic bacteria) using different carcasses from the same flock and also using different analytical units taken from the same carcass. Eighty flocks, with four broiler carcasses from each, were included in this study; three broilers were sampled according to protocols from Brazil, the EU, and the United States, and the last one by all three methods. SME, NSE, and WCR provided equivalent results (P > 0.05) for Salmonella detection on broiler carcasses when using different carcasses from the same flock and when using the same carcass. The predominant serovar was Salmonella Enteritidis. For the enumeration of hygiene indicator microorganisms, WRC provided higher counts than SME or NSE (P < 0.05), when using both the same or different carcasses. Therefore, it is possible to directly compare Salmonella results in poultry carcasses when using the methods recommended by the legislative bodies of Brazil, the United States, and the EU. However, WCR provides the best results for hygiene indicator microorganisms.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella / Contaminação de Alimentos / Manipulação de Alimentos / Imersão / Carne Limite: Animals / Humans País/Região como assunto: America do norte / America do sul / Brasil Idioma: En Revista: J Food Prot Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Salmonella / Contaminação de Alimentos / Manipulação de Alimentos / Imersão / Carne Limite: Animals / Humans País/Região como assunto: America do norte / America do sul / Brasil Idioma: En Revista: J Food Prot Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos