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Effects of gravitational perturbation on the expression of genes regulating metabolism in Jurkat cells.
Singh, Kanika; Cubano, Luis; Lewis, Marian.
Afiliação
  • Singh K; Department of Biological Sciences, University of Alabama, Hunstville, USA
Bol Asoc Med P R ; 102(3): 21-6, 2010.
Article em En | MEDLINE | ID: mdl-23875517
Gravitational pertubation altered gene expression and increased glucose consumption in spaceflown Jurkat cells. The purpose of this study was to determine if the acceleration experienced during launch was responsible for these changes. In ground-based studies, cells were subjected to typical launch centrifugal acceleration (3g of force for eight minutes) and centrifugal force of 90g for five minutes (commonly used to sediment cells) in a laboratory centrifuge. Controls consisted of static cultures. Gene expression was analyzed by RT-PCR. pH and glucose concentrations were evaluated to monitor metabolic changes. Comparison with controls indicated no significant change in pH or glucose use. Gene expression of Jurkat cells subjected to 3g or 90g of force was altered for only two genes out of seven tested. This research suggests that the changes observed in Jurkat cells flown on STS-95 were not a result of launch acceleration but to other conditions experienced during space flight.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Expressão Gênica / Células Jurkat / Gravitação Limite: Humans Idioma: En Revista: Bol Asoc Med P R Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Porto Rico

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Expressão Gênica / Células Jurkat / Gravitação Limite: Humans Idioma: En Revista: Bol Asoc Med P R Ano de publicação: 2010 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Porto Rico