A reliable method to quantify sildenafil and its metabolite N-demethylsildenafil by HPLC in plasma of children.
Drug Res (Stuttg)
; 63(9): 473-6, 2013 Sep.
Article
em En
| MEDLINE
| ID: mdl-23737285
The aim of this study was to develop a simple method for quantifying plasma levels of sildenafil and its metabolite by liquid chromatography with a C18 reverse-phase column and UV detection. For both compounds, linearity was assessed in the range from 10 and 1 000 ng · ml-1 and had correlation coefficients of r=0.995 and r=0.997 for sildenafil and its metabolite, respectively. The inter- and intra-day coefficients of variation was<5.3%. The limits of detection and quantification were 1 and 10 ng · ml-1. Drug levels were determined satisfactorily in two patients. A simple and reliable method was developed for use in children with Pulmonary Arterial Hypertension under treatment with sildenafil.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Piperazinas
/
Sulfonas
/
Cromatografia Líquida de Alta Pressão
/
Inibidores da Fosfodiesterase 5
Limite:
Child
/
Humans
Idioma:
En
Revista:
Drug Res (Stuttg)
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Alemanha