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Vitamin D3 induces IDO+ tolerogenic DCs and enhances Treg, reducing the severity of EAE.
Farias, Alessandro S; Spagnol, Gabriela S; Bordeaux-Rego, Pedro; Oliveira, Camila O F; Fontana, Ana Gabriela M; de Paula, Rosemeire F O; Santos, Mariana P A; Pradella, Fernando; Moraes, Adriel S; Oliveira, Elaine C; Longhini, Ana Leda F; Rezende, Alexandre C S; Vaisberg, Mauro W; Santos, Leonilda M B.
Afiliação
  • Farias AS; Neuroimmunology Unit, Department of Genetics, Evolution and Bioagents, University of Campinas (UNICAMP), Campinas, SP, Brazil.
CNS Neurosci Ther ; 19(4): 269-77, 2013 Apr.
Article em En | MEDLINE | ID: mdl-23521914
BACKGROUND: A growing body of evidence supports the hypothesis that vitamin D is an important environmental factor in the etiology of T-cell-mediated autoimmune diseases such as multiple sclerosis (MS). AIM: The purpose of this study was exploring the mechanisms underlying the beneficial effect of vitamin D3 in encephalomyelitis (EAE). METHODS: We treated monophasic experimental autoimmune EAE, induced in Lewis rat, with vitamin D3 and adoptively transfer tolerogenic bone marrow-derived DCs generated in the presence of vitamin D3. RESULTS: This study provides evidence that the in vivo administration of vitamin D3, as well as the adoptive transfer of vitamin D3 -induced IDO(+) immature/tolerogenic dendritic cells, leads to a significant increase in the percentage of CD4(+) CD25(+) Foxp3(+) regulatory T cells in the lymph nodes in a rat model of MS, experimental autoimmune EAE. Concomitant with the increase in this cell population, there is a significant decrease in the number of autoreactive T cells in the central nervous system. Bone marrow-derived DCs cultivated in the presence of vitamin D3 present a tolerogenic profile with high IL-10, TNFα, and IDO expression and decreased MHC-II and CD80 expression. The adoptive transfer of IDO (+) DCs induces a significant increase in the percentage of CD4(+) CD25(+) Foxp3(+) T cells in the lymph nodes, comparable with vitamin D3 treatment. CONCLUSION: These mechanisms contribute actively to the generation of a microenvironment in the lymph nodes that suppresses the activation of encephalitogenic T cells, resulting in the downregulation of the inflammatory response in the central nervous system.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Índice de Gravidade de Doença / Células Dendríticas / Linfócitos T Reguladores / Colecalciferol / Encefalomielite Autoimune Experimental Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: CNS Neurosci Ther Assunto da revista: NEUROLOGIA / TERAPEUTICA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Índice de Gravidade de Doença / Células Dendríticas / Linfócitos T Reguladores / Colecalciferol / Encefalomielite Autoimune Experimental Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: CNS Neurosci Ther Assunto da revista: NEUROLOGIA / TERAPEUTICA Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido