Bcl-3 regulates UVB-induced apoptosis.
Hum Cell
; 26(2): 47-55, 2013 Jun.
Article
em En
| MEDLINE
| ID: mdl-23494744
B cell leukemia-3 (Bcl-3) has been defined as an anti-apoptotic gene; however, the exact mechanisms through which Bcl-3 influences apoptosis have been elusive. To determine the specific role of Bcl-3 in apoptosis, we evaluated the effect of its silencing on the expression of proteins involved in either the extrinsic or intrinsic apoptotic pathways induced by ultraviolet light B-mediated DNA damage. We found that, in Bcl-3-silenced cells, caspase-3, caspase-8 and caspase-9 activation is accelerated and tBid mitochondrial content is increased. It is important to note that, although mitochondrial Smac levels were reduced after UV exposure, the rate of reduction was slightly higher in Bcl-3 silenced cells than in control cells. Additionally, p53 levels diminished in Bcl-3 silenced cells compared to control cells, as did those of DNA-PK, a DNA repair protein. Altogether, our data indicate that Bcl-3 protects cells from apoptosis by regulating both apoptotic pathways.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Raios Ultravioleta
/
Proteínas Proto-Oncogênicas
/
Apoptose
Limite:
Humans
Idioma:
En
Revista:
Hum Cell
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Japão