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[Aberrant immunophenotypes in acute leukemia in a Buenos Aires' hospital population]. / Inmunofenotipos aberrantes en leucemias agudas en una población hospitalaria de Buenos Aires.
Novoa, Viviana; Núñez, Neri A; Carballo, Orlando G; Lessa, Carmen F.
Afiliação
  • Novoa V; Laboratorio de Inmunología, Unidad de Inmunología e Histocompatibilidad, Hospital de Agudos Dr. Carlos G. Durand, Buenos Aires, Argentina. novoaviviana@yahoo.com.ar
Medicina (B Aires) ; 73(1): 9-16, 2013.
Article em Es | MEDLINE | ID: mdl-23335699
Multiparameter flow cytometry (MFC) has become the preferred method for the lineage assignment and maturational analysis of malignant cells in acute leukemias. Multiparametric immunophenotyping analysis allows the detection of aberrant antigen expression and the analysis of heterogeneity and clonality of malignant cells in leukemias. Our objectives were to analyze the membrane antigen expression and to evaluate if the aberrant phenotypes occurrence in blasts cells of patients with acute leukemia is useful in monitoring the response to the treatment. We have retrospectively analyzed the MFC data of 364 samples sent to our laboratory in a 7 years period. For this purpose we have used a large panel of monoclonal antibodies against lymphoid, myeloid and precursors antigens. From the 364 analyzed samples, 60.2% showed a phenotype compatible with acute myeloid leukemia (AML), 28.8% with B lymphoblastic leukemia (B-LLA), 6.6% with T lymphoblastic leukemia (T-LLA) and 4.4% with rare leukemias. Aberrant phenotypes were found in 86% of the samples. The aberrant phenotypes identified were:1) lineage infidelity AML (54%), B-ALL (40%), T-ALL (29%); 2) absence of antigen expression: AML (21%), B-ALL (35%), T-ALL (70%); 3) altered antigen expression: AML (67%), B-ALL (66%),T-ALL (84%); 4) asynchronous expression: AML (26%), B-ALL (37%) and 5) ectopic phenotype: T-ALL (96%). Multiparameter flow cytometry of acute leukemias allowed identification of aberrant phenotypes in the majority of our patients, that are helpful for monitoring treatment response.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucemia / Antígenos CD / Imunofenotipagem Tipo de estudo: Observational_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Argentina Idioma: Es Revista: Medicina (B Aires) Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Argentina País de publicação: Argentina
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leucemia / Antígenos CD / Imunofenotipagem Tipo de estudo: Observational_studies Limite: Adolescent / Adult / Aged / Aged80 / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Argentina Idioma: Es Revista: Medicina (B Aires) Ano de publicação: 2013 Tipo de documento: Article País de afiliação: Argentina País de publicação: Argentina