Numerical evaluation of the limit of concentration of colloidal samples for their study with digital lensless holographic microscopy.
Appl Opt
; 52(1): A310-8, 2013 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-23292407
The number of colloidal particles per unit of volume that can be imaged correctly with digital lensless holographic microscopy (DLHM) is determined numerically. Typical in-line DLHM holograms with controlled concentration are modeled and reconstructed numerically. By quantifying the ratio of the retrieved particles from the reconstructed hologram to the number of the seeding particles in the modeled intensity, the limit of concentration of the colloidal suspensions up to which DLHM can operate successfully is found numerically. A new shadow density parameter for spherical illumination is defined. The limit of performance of DLHM is determined from a graph of the shadow density versus the efficiency of the microscope.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processamento de Sinais Assistido por Computador
/
Holografia
/
Interpretação de Imagem Assistida por Computador
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Coloides
/
Nanopartículas
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Appl Opt
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Colômbia
País de publicação:
Estados Unidos