A set of plastid microsatellite loci for the genus Dyckia (Bromeliaceae) derived from 454 pyrosequencing.
Am J Bot
; 99(12): e470-3, 2012 Dec.
Article
em En
| MEDLINE
| ID: mdl-23196399
PREMISE OF THE STUDY: Phylogeographical analyses of Dyckia (Bromeliaceae) suffer from low levels of sequence variation. Plastid microsatellite markers were developed to achieve a better-resolved genus-wide plastid genealogy of Dyckia. ⢠METHODS AND RESULTS: Approximately 84% of the D. marnier-lapostollei plastome was sequenced using 454 technology. Flanking primer pairs were designed for 34 out of 36 chloroplast simple sequence repeats (cpSSRs) detected, and 12 loci were further characterized by genotyping Dyckia samples at the level of populations and species. Three, five, and six cpSSRs were polymorphic among four individuals of D. limae, 12 individuals of D. dissitiflora, and 12 of D. pernambucana, respectively, with two to three alleles per locus and species. All loci were polymorphic among 19 different Dyckia species, with three to 10 alleles per locus. Ten primer pairs cross-amplified with bromeliad genera from five subfamilies. ⢠CONCLUSIONS: The set of 12 cpSSR markers provides a toolbox to analyze phylogeographical patterns of Dyckia species.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polimorfismo Genético
/
Cloroplastos
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Primers do DNA
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DNA de Plantas
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Repetições de Microssatélites
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Bromeliaceae
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Am J Bot
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Alemanha
País de publicação:
Estados Unidos