Titanium dioxide nanoparticles inhibit proliferation and induce morphological changes and apoptosis in glial cells.
Toxicology
; 302(2-3): 146-56, 2012 Dec 16.
Article
em En
| MEDLINE
| ID: mdl-23044362
Titanium dioxide nanoparticles (TiO(2) NPs) are widely used in the chemical, electrical and electronic industries. TiO(2) NPs can enter directly into the brain through the olfactory bulb and be deposited in the hippocampus region. We determined the effect of TiO(2) NPs on rat and human glial cells, C6 and U373, respectively. We evaluated proliferation by crystal violet staining, internalization of TiO(2) NPs, and cellular morphology by TEM analysis, as well as F-actin distribution by immunostaining and cell death by detecting active caspase-3 and DNA fragmentation. TiO(2) NPs inhibited proliferation and induced morphological changes that were related with a decrease in immuno-location of F-actin fibers. TiO(2) NPs were internalized and formation of vesicles was observed. TiO(2) NPs induced apoptosis after 96h of treatment. Hence, TiO(2) NPs had a cytotoxic effect on glial cells, suggesting that exposure to TiO(2) NPs could cause brain injury and be hazardous to health.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Titânio
/
Neuroglia
/
Apoptose
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Proliferação de Células
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Nanopartículas
Limite:
Animals
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Humans
Idioma:
En
Revista:
Toxicology
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
México
País de publicação:
Irlanda