Optimization of dengue virus genome assembling using GSFLX 454 pyrosequencing data: evaluation of assembling strategies.
Genet Mol Res
; 11(4): 3688-95, 2012 Oct 11.
Article
em En
| MEDLINE
| ID: mdl-22930429
Currently assembling genomes without reference is one of the most important challenges for bioinformaticists all over the world in an attempt to characterize new organisms. The current study has used two dengue virus type 4 (DENV-4) strains recently isolated in Brazil, which have its genomes sequenced using the GSFLX 454 sequencer (Roche, Life Science) by the pyrosequencing method. The GSFLX 454 data were used for testing different genome assembling strategies. We described a pipeline that was able to recover more than 96% of the sequenced genome in a single run and could be helpful for further assembly attempts of other DENV genomes, as well as other RNA virus-like genomes.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estatística como Assunto
/
Genoma Viral
/
Análise de Sequência de DNA
/
Biologia Computacional
/
Vírus da Dengue
Idioma:
En
Revista:
Genet Mol Res
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil