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Decreased erythrocyte NA+,K+-ATPase activity and increased plasma TBARS in prehypertensive patients.
Malfatti, Carlos Ricardo Maneck; Burgos, Leandro Tibiriçá; Rieger, Alexandre; Rüdger, Cássio Luiz; Túrmina, Janaína Angela; Pereira, Ricardo Aparecido; Pavlak, João Lang; Silva, Luiz Augusto; Osiecki, Raul.
Afiliação
  • Malfatti CR; Department of Physical Education, Midwest State University, Campus Irati, 84500-000 PR, Irati, Brazil. crmalfatti@gmail.com
ScientificWorldJournal ; 2012: 348246, 2012.
Article em En | MEDLINE | ID: mdl-22919304
The essential hypertension has been associated with membrane cell damage. The aim of the present study is investigate the relationship between erythrocyte Na(+),K(+)-ATPase and lipoperoxidation in prehypertensive patients compared to normotensive status. The present study involved the prehypertensive patients (systolic: 136 ± 7 mmHg; diastolic: 86.8 ± 6.3 mmHg; n = 8) and healthy men with normal blood pressure (systolic: 110 ± 6.4 mmHg; diastolic: 76.1 ± 4.2 mmHg; n = 8) who were matched for age (35 ± 4 years old). The venous blood samples of antecubital vein (5 mL) were collected into a tube containing sodium heparin as anticoagulant (1000 UI), and erythrocyte ghosts were prepared for quantifying Na(+),K(+)-ATPase activity. The extent of the thiobarbituric acid reactive substances (TBARS) was determined in plasma. The statistical analysis was carried out by Student's t-test and Pearson's correlation coefficient. A P < 0.05 was considered significant. The Na(+),K(+)-ATPase activity was lower in prehypertensive patients compared with normotensive subjects (4.9 versus 8.0 nmol Pi/mg protein/min; P < 0.05). The Na(+),K(+)-ATPase activity correlated negatively with TBARS content (r = -0.6; P < 0.05) and diastolic blood pressure (r = -0.84; P < 0.05). The present study suggests that Na(+),K(+)-ATPase activity reduction and elevation of the TBARS content may underlie the pathophysiological aspects linked to the prehypertensive status.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Substâncias Reativas com Ácido Tiobarbitúrico / ATPase Trocadora de Sódio-Potássio / Eritrócitos / Pré-Hipertensão Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans / Male Idioma: En Revista: ScientificWorldJournal Assunto da revista: MEDICINA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Substâncias Reativas com Ácido Tiobarbitúrico / ATPase Trocadora de Sódio-Potássio / Eritrócitos / Pré-Hipertensão Tipo de estudo: Observational_studies / Risk_factors_studies Limite: Humans / Male Idioma: En Revista: ScientificWorldJournal Assunto da revista: MEDICINA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos