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Influence of cellular fatty acid composition on the response of Saccharomyces cerevisiae to hydrostatic pressure stress.
de Freitas, Jéssica M; Bravim, Fernanda; Buss, David S; Lemos, Elenice M; Fernandes, A Alberto R; Fernandes, Patricia M B.
Afiliação
  • de Freitas JM; Núcleo de Biotecnologia, Centro de Ciências da Saúde, Universidade Federal do Espírito Santo, Vitória, ES, Brazil.
FEMS Yeast Res ; 12(8): 871-8, 2012 Dec.
Article em En | MEDLINE | ID: mdl-22846157
High hydrostatic pressure (HHP) interferes with cellular membrane structure. The orientation of lipid molecules is changed, especially in the vicinity of proteins, leading to decreased membrane fluidity. Adaptation to HHP requires increased membrane fluidity, often achieved through a rise in the proportion of unsaturated fatty acids. In this work, a desaturase-deficient Saccharomyces cerevisiae mutant strain (OLE1 gene deletion) was grown in media supplemented with fatty acids differing in size and number of unsaturations and submitted to pressure up to 200 MPa for 30 min. Desaturase-deficient yeast supplemented with palmitoleic acid demonstrated increased sensitivity to pressure compared to cells supplemented with oleic acid or a proportionate mixture of both acids. In contrast, yeast cells grown with linoleic and linolenic acids were more piezoresistant than cells treated with oleic acid. Furthermore, growth with palmitoleic acid led to higher levels of lipid peroxidation. Intracellular trehalose during HHP treatment increased cell tolerance to pressure. However, when trehalose remained extracellular cells were sensitised to pressure. Therefore, fatty acid composition and trehalose content might play a role in the protection of the cell membrane from oxidative damage produced by HHP, confirming that alteration in cell membrane fluidity is correlated with pressure resistance in yeast.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Estresse Fisiológico / Membrana Celular / Ácido alfa-Linolênico / Ácido Linoleico Idioma: En Revista: FEMS Yeast Res Assunto da revista: MICROBIOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Estresse Fisiológico / Membrana Celular / Ácido alfa-Linolênico / Ácido Linoleico Idioma: En Revista: FEMS Yeast Res Assunto da revista: MICROBIOLOGIA Ano de publicação: 2012 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido