Effect of tamoxifen on extracellular signal-regulated kinases in the urethra of castrated female rats.
Eur J Obstet Gynecol Reprod Biol
; 164(1): 102-4, 2012 Sep.
Article
em En
| MEDLINE
| ID: mdl-22721636
OBJECTIVE: The aim was to evaluate the effects of tamoxifen in activating extracellular signal-regulated kinases (ERKs) 1 and 2 in the urethras of castrated female rats. STUDY DESIGN: Twelve castrated adult female rats were divided into a control group (n=6) in which the animals received vehicle, and the experimental group (n=6) in which the rats received tamoxifen 250 µg/day by gavage for 28 days. Then, the animals were sacrificed and their urethras removed. Proteins were extracted, quantified and processed by Western blot analysis with specific phospho-ERK1 and 2 antibodies. Data were analyzed using Student's t-test (p<0.05). RESULTS: A significant increase occurred in phospho-ERK1 levels in the experimental group compared to the control group (p<0.01), while no difference was found in phospho-ERK2 levels between the groups (p=0.313). CONCLUSION: The present results indicate that, at the doses and during the time of treatment used, tamoxifen significantly increased phospho-ERK1 levels in the urethras of castrated female rats.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tamoxifeno
/
Uretra
/
Proteína Quinase 1 Ativada por Mitógeno
/
MAP Quinases Reguladas por Sinal Extracelular
/
Proteína Quinase 3 Ativada por Mitógeno
Limite:
Animals
Idioma:
En
Revista:
Eur J Obstet Gynecol Reprod Biol
Ano de publicação:
2012
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Irlanda