[Fluorescent method (fluorescein diacetate and ethidium bromide) to study the viability of Cryptococcus neoformans in liquor]. / Método fluorescente (diacetato de fluoresceína e brometo de etídio) para o estudo da viabilidade de Cryptococcus neoformans em líquor.
Rev Inst Med Trop Sao Paulo
; 32(1): 46-50, 1990.
Article
em Pt
| MEDLINE
| ID: mdl-2259831
The utilization of the fluorescent method (fluorescein diacetate DF and ethidium bromide BE), to verify the viability of fungal cells, was studied in 40 samples of liquor, from patients with neurocryptococcosis. For removing leukocytes and red blood cells, which produce interfering fluorescence, good results were obtained with 0.3% saponin solution. After processing of liquor, 0.1 ml aliquots of resulting suspension were mixed to equal volumes of fresh DF-BE solution. The best incubation period for staining was 30 minutes, resulting in good differentiation between viable (green fluorescence) and non viable (red fluorescence) cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Líquido Cefalorraquidiano
/
Cryptococcus neoformans
/
Etídio
/
Fluoresceínas
Limite:
Humans
Idioma:
Pt
Revista:
Rev Inst Med Trop Sao Paulo
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
Brasil